biostudies-literatureJiang TNational Natural Science Foundation of Chinauhae129https://www.ebi.ac.uk/biostudies/studies/S-EPMC11220176biostudies-literatureUnknown11(7)Long non-coding RNAs (lncRNAs) have gathered significant attention due to their pivotal role in plant growth, development, and biotic and abiotic stress resistance. Despite this, there is still little understanding regarding the functions of lncRNA in these domains in the tea plant (<i>Camellia sinensis</i>), mainly attributable to the insufficiencies in gene manipulation techniques for tea plants. In this study, we designed a novel strategy to identify evolutionarily conserved <i>trans</i>-lncRNA (ECT-lncRNA) pairs in plants. We used highly consistent base sequences in the exon-overlapping region between <i>trans</i>-lncRNAs and their target gene transcripts. Based on this method, we successfully screened 24 ECT-lncRNA pairs from at least two or more plant species. In tea, as observed in model plants such as <i>Arabidopsis</i>, alfalfa, potatoes, and rice, there exists a <i>trans</i>-lncRNA capable of forming an ECT-lncRNA pair with transcripts of the 12-oxophytodienoate reductase (<i>OPR</i>) family, denoted as the <i>OPRL/OPR</i> pair. Considering evolutionary perspectives, the <i>OPRL</i> gene cluster in each species likely originates from a replication event of the <i>OPR</i> gene cluster. Gene manipulation and gene expression analysis revealed that <i>CsOPRL</i> influences disease resistance by regulating <i>CsOPR</i> expression in tea plants. Furthermore, the knockout of <i>StOPRL1</i> in <i>Solanum tuberosum</i> led to aberrant growth characteristics and strong resistance to fungal infection. This study provides insights into a strategy for the screening and functional verification of ECT-lncRNA pairs.Horticulture researchEvolutionarily conserved 12-oxophytodienoate reductase <i>trans</i>-lncRNA pair affects disease resistance in tea (<i>Camellia sinensis</i>) via the jasmonic acid signaling pathway.PMC1122017632372756, U21A20232Liu YHu YLi TJiang XJiao TJiang TLiu CXia TGao LPfalseEvolutionarily conserved 12-oxophytodienoate reductase <i>trans</i>-lncRNA pair affects disease resistance in tea (<i>Camellia sinensis</i>) via the jasmonic acid signaling pathway.Long non-coding RNAs (lncRNAs) have gathered significant attention due to their pivotal role in plant growth, development, and biotic and abiotic stress resistance. Despite this, there is still little understanding regarding the functions of lncRNA in these domains in the tea plant (<i>Camellia sinensis</i>), mainly attributable to the insufficiencies in gene manipulation techniques for tea plants. In this study, we designed a novel strategy to identify evolutionarily conserved <i>trans</i>-lncRNA (ECT-lncRNA) pairs in plants. We used highly consistent base sequences in the exon-overlapping region between <i>trans</i>-lncRNAs and their target gene transcripts. Based on this method, we successfully screened 24 ECT-lncRNA pairs from at least two or more plant species. In tea, as observed in model plants such as <i>Arabidopsis</i>, alfalfa, potatoes, and rice, there exists a <i>trans</i>-lncRNA capable of forming an ECT-lncRNA pair with transcripts of the 12-oxophytodienoate reductase (<i>OPR</i>) family, denoted as the <i>OPRL/OPR</i> pair. Considering evolutionary perspectives, the <i>OPRL</i> gene cluster in each species likely originates from a replication event of the <i>OPR</i> gene cluster. Gene manipulation and gene expression analysis revealed that <i>CsOPRL</i> influences disease resistance by regulating <i>CsOPR</i> expression in tea plants. Furthermore, the knockout of <i>StOPRL1</i> in <i>Solanum tuberosum</i> led to aberrant growth characteristics and strong resistance to fungal infection. This study provides insights into a strategy for the screening and functional verification of ECT-lncRNA pairs.2024-01-01T00:00:00Z2024 Jul2026-04-21T03:21:00.384Z2025-04-04T12:21:59.709ZS-EPMC112201763896686510.1093/hr/uhae129