{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Krieger MR"],"funding":["HHS | NIH | National Institute of General Medical Sciences","HHS | NIH | National Cancer Institute","NCI NIH HHS","NIGMS NIH HHS"],"pagination":["e202302396"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11220484"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(9)"],"pubmed_abstract":["In addition to mitochondrial DNA, mitochondrial double-stranded RNA (mtdsRNA) is exported from mitochondria. However, specific channels for RNA transport have not been demonstrated. Here, we begin to characterize channel candidates for mtdsRNA export from the mitochondrial matrix to the cytosol. Down-regulation of SUV3 resulted in the accumulation of mtdsRNAs in the matrix, whereas down-regulation of PNPase resulted in the export of mtdsRNAs to the cytosol. Targeting experiments show that PNPase functions in both the intermembrane space and matrix. Strand-specific sequencing of the double-stranded RNA confirms the mitochondrial origin. Inhibiting or down-regulating outer membrane proteins VDAC1/2 and BAK/BAX or inner membrane proteins PHB1/2 strongly attenuated the export of mtdsRNAs to th"],"journal":["Life science alliance"],"pubmed_title":["Trafficking of mitochondrial double-stranded RNA from mitochondria to the cytosol."],"pmcid":["PMC11220484"],"funding_grant_id":["CA208642, CA267721","R01 CA267721","GM073981","R01 GM073981","GM61721","R01 CA208642","R01 GM061721"],"pubmed_authors":["Krieger MR","He KL","Chanfreau GF","Momcilovic M","Shackelford DB","Koehler CM","Abrahamian M","Maggo SD","Tsang YP","Teitell MA","Atamdede S","Gai X","Hakimjavadi H","Ostrow D"],"additional_accession":[]},"is_claimable":false,"name":"Trafficking of mitochondrial double-stranded RNA from mitochondria to the cytosol.","description":"In addition to mitochondrial DNA, mitochondrial double-stranded RNA (mtdsRNA) is exported from mitochondria. However, specific channels for RNA transport have not been demonstrated. Here, we begin to characterize channel candidates for mtdsRNA export from the mitochondrial matrix to the cytosol. Down-regulation of SUV3 resulted in the accumulation of mtdsRNAs in the matrix, whereas down-regulation of PNPase resulted in the export of mtdsRNAs to the cytosol. Targeting experiments show that PNPase functions in both the intermembrane space and matrix. Strand-specific sequencing of the double-stranded RNA confirms the mitochondrial origin. Inhibiting or down-regulating outer membrane proteins VDAC1/2 and BAK/BAX or inner membrane proteins PHB1/2 strongly attenuated the export of mtdsRNAs to th","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2025-04-22T12:02:56.036Z","creation":"2025-04-06T00:14:05.226Z"},"accession":"S-EPMC11220484","cross_references":{"pubmed":["38955468"],"doi":["10.26508/lsa.202302396"]}}