biostudies-literatureHe Fthe Key Program of Sichuan Science and Technology Department424https://www.ebi.ac.uk/biostudies/studies/S-EPMC11220950biostudies-literatureUnknown24(1)<h4>Objectives</h4>Although recent discoveries regarding the biomarkers of newborn screening (NBS) programs by tandem mass spectrometry (MS/MS) highlight the critical need to establish reference intervals (RIs) specifically for preterm infants, no such RIs has been formally published yet. This study addressed the gap by offering a comprehensive set of reference intervals (RIs) for preterm neonates, and illustrating the dynamic changes of each biomarker with age.<h4>Design and methods</h4>The NBS data of 199,693 preterm newborns (< 37 weeks of gestation) who met the inclusion and exclusion criteria from the NNSCP database were included in study analysis. The birth weight stratified dynamic trend of each biomarker were captured by their concentrations over age. Reference partitions were deteBMC pediatricsRetrospective analysis of reference intervals for dried blood spot based ms/ms newborn screening programs in Chinese preterm neonates: a nationwide study.PMC11220950No. 2021YFS0179 & No. 2021YFQ0060No. 2021YFS0179 &amp; No. 2021YFQ0060He FXie TZhang JHuang XTang TfalseRetrospective analysis of reference intervals for dried blood spot based ms/ms newborn screening programs in Chinese preterm neonates: a nationwide study.<h4>Objectives</h4>Although recent discoveries regarding the biomarkers of newborn screening (NBS) programs by tandem mass spectrometry (MS/MS) highlight the critical need to establish reference intervals (RIs) specifically for preterm infants, no such RIs has been formally published yet. This study addressed the gap by offering a comprehensive set of reference intervals (RIs) for preterm neonates, and illustrating the dynamic changes of each biomarker with age.<h4>Design and methods</h4>The NBS data of 199,693 preterm newborns (< 37 weeks of gestation) who met the inclusion and exclusion criteria from the NNSCP database were included in study analysis. The birth weight stratified dynamic trend of each biomarker were captured by their concentrations over age. Reference partitions were dete2024-01-01T00:00:00Z2024 Jul2025-04-06T00:12:55.897Z2025-04-06T00:12:55.897ZS-EPMC112209503895649410.1186/s12887-024-04865-1