biostudies-literatureZhu YNational Natural Science Foundation of ChinaShanghai Sailing Program of Science and Technology Commission of Shanghai MunicipalityChenguang Program from the Shanghai Education Committee612https://www.ebi.ac.uk/biostudies/studies/S-EPMC11221097biostudies-literatureUnknown22(1)<h4>Background</h4>Programmed cell death (PCD) has recently been implicated in modulating the removal of neutrophils recruited in acute myocardial infarction (AMI). Nonetheless, the clinical significance and biological mechanism of neutrophil-related PCD remain unexplored.<h4>Methods</h4>We employed an integrative machine learning-based computational framework to generate a predictive neutrophil-derived PCD signature (NPCDS) within five independent microarray cohorts from the peripheral blood of AMI patients. Non-negative matrix factorization was leveraged to develop an NPCDS-based AMI subtype. To elucidate the biological mechanism underlying NPCDS, we implemented single-cell transcriptomics on Cd45+ cells isolated from the murine heart of experimental AMI. We finally conducted a MendelianJournal of translational medicineLeveraging a neutrophil-derived PCD signature to predict and stratify patients with acute myocardial infarction: from AI prediction to biological interpretation.PMC11221097315011663217042315YF140500014CG49Zhu YChen YZu YfalseLeveraging a neutrophil-derived PCD signature to predict and stratify patients with acute myocardial infarction: from AI prediction to biological interpretation.<h4>Background</h4>Programmed cell death (PCD) has recently been implicated in modulating the removal of neutrophils recruited in acute myocardial infarction (AMI). Nonetheless, the clinical significance and biological mechanism of neutrophil-related PCD remain unexplored.<h4>Methods</h4>We employed an integrative machine learning-based computational framework to generate a predictive neutrophil-derived PCD signature (NPCDS) within five independent microarray cohorts from the peripheral blood of AMI patients. Non-negative matrix factorization was leveraged to develop an NPCDS-based AMI subtype. To elucidate the biological mechanism underlying NPCDS, we implemented single-cell transcriptomics on Cd45+ cells isolated from the murine heart of experimental AMI. We finally conducted a Mendelian2024-01-01T00:00:00Z2024 Jul2026-07-03T03:17:34.653Z2024-10-16T07:26:13.837ZS-EPMC112210973895666910.1186/s12967-024-05415-0