biostudies-literatureDe Leonardis MHorizon 2020 Framework ProgrammeCentro Nazionale di Ricerca in High Performance Computing, Big Data, and Quantum ComputingFuture Artificial Intelligence Research229https://www.ebi.ac.uk/biostudies/studies/S-EPMC11221173biostudies-literatureUnknown25(1)Adeno-associated viruses 2 (AAV2) are minute viruses renowned for their capacity to infect human cells and akin organisms. They have recently emerged as prominent candidates in the field of gene therapy, primarily attributed to their inherent non-pathogenic nature in humans and the safety associated with their manipulation. The efficacy of AAV2 as gene therapy vectors hinges on their ability to infiltrate host cells, a phenomenon reliant on their competence to construct a capsid capable of breaching the nucleus of the target cell. To enhance their infection potential, researchers have extensively scrutinized various combinatorial libraries by introducing mutations into the capsid, aiming to boost their effectiveness. The emergence of high-throughput experimental techniques, like deep mutatBMC bioinformaticsUnsupervised modeling of mutational landscapes of adeno-associated viruses viability.PMC11221173734439De Leonardis MFernandez-de-Cossio-Diaz JPagnani AUguzzoni GfalseUnsupervised modeling of mutational landscapes of adeno-associated viruses viability.Adeno-associated viruses 2 (AAV2) are minute viruses renowned for their capacity to infect human cells and akin organisms. They have recently emerged as prominent candidates in the field of gene therapy, primarily attributed to their inherent non-pathogenic nature in humans and the safety associated with their manipulation. The efficacy of AAV2 as gene therapy vectors hinges on their ability to infiltrate host cells, a phenomenon reliant on their competence to construct a capsid capable of breaching the nucleus of the target cell. To enhance their infection potential, researchers have extensively scrutinized various combinatorial libraries by introducing mutations into the capsid, aiming to boost their effectiveness. The emergence of high-throughput experimental techniques, like deep mutat2024-01-01T00:00:00Z2024 Jul2025-04-04T12:54:55.396Z2025-04-04T12:54:55.396ZS-EPMC112211733895647410.1186/s12859-024-05823-5