{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jekhmane S"],"funding":["Netherlands Centre for One Health","Dutch Research Council (NWO)","EC | Horizon 2020 Framework Programme","EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020)"],"pagination":["1778-1791"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11222147"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(7)"],"pubmed_abstract":["Antimicrobial resistance is a leading cause of mortality, calling for the development of new antibiotics. The fungal antibiotic plectasin is a eukaryotic host defence peptide that blocks bacterial cell wall synthesis. Here, using a combination of solid-state nuclear magnetic resonance, atomic force microscopy and activity assays, we show that plectasin uses a calcium-sensitive supramolecular killing mechanism. Efficient and selective binding of the target lipid II, a cell wall precursor with an irreplaceable pyrophosphate, is achieved by the oligomerization of plectasin into dense supra-structures that only form on bacterial membranes that comprise lipid II. Oligomerization and target binding of plectasin are interdependent and are enhanced by the coordination of calcium ions to plectasin'"],"journal":["Nature microbiology"],"pubmed_title":["Host defence peptide plectasin targets bacterial cell wall precursor lipid II by a calcium-sensitive supramolecular mechanism."],"pmcid":["PMC11222147"],"funding_grant_id":["711.018.001","101045485","184.035.002","184.032.207","101008500","723.014.003"],"pubmed_authors":["Slingerland CJ","Kunzler M","Lelli M","Matheson EJ","Cochrane RVK","Lorent JH","Medeiros-Silva J","Cochrane SA","Jekhmane S","Charitou V","Lavore F","Consoli NA","Derks MGN","Maity S","Roos WH","Breukink E","Baldus M","Weingarth M","Fetz C","Tehrani KHME","Ammerlaan D","van der Weijde M","Elenbaas BOW","Cox R","Martin NI"],"additional_accession":[]},"is_claimable":false,"name":"Host defence peptide plectasin targets bacterial cell wall precursor lipid II by a calcium-sensitive supramolecular mechanism.","description":"Antimicrobial resistance is a leading cause of mortality, calling for the development of new antibiotics. The fungal antibiotic plectasin is a eukaryotic host defence peptide that blocks bacterial cell wall synthesis. Here, using a combination of solid-state nuclear magnetic resonance, atomic force microscopy and activity assays, we show that plectasin uses a calcium-sensitive supramolecular killing mechanism. Efficient and selective binding of the target lipid II, a cell wall precursor with an irreplaceable pyrophosphate, is achieved by the oligomerization of plectasin into dense supra-structures that only form on bacterial membranes that comprise lipid II. Oligomerization and target binding of plectasin are interdependent and are enhanced by the coordination of calcium ions to plectasin'","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2025-04-04T12:55:23.731Z","creation":"2025-04-04T12:55:23.731Z"},"accession":"S-EPMC11222147","cross_references":{"pubmed":["38783023"],"doi":["10.1038/s41564-024-01696-9"]}}