{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zheng W"],"funding":["NIGMS NIH HHS","NIH HHS"],"pagination":["232-239"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11222160"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["631(8019)"],"pubmed_abstract":["Mitochondria play a pivotal part in ATP energy production through oxidative phosphorylation, which occurs within the inner membrane through a series of respiratory complexes<sup>1-4</sup>. Despite extensive in vitro structural studies, determining the atomic details of their molecular mechanisms in physiological states remains a major challenge, primarily because of loss of the native environment during purification. Here we directly image porcine mitochondria using an in situ cryo-electron microscopy approach. This enables us to determine the structures of various high-order assemblies of respiratory supercomplexes in their native states. We identify four main supercomplex organizations: I<sub>1</sub>III<sub>2</sub>IV<sub>1</sub>, I<sub>1</sub>III<sub>2</sub>IV<sub>2</sub>, I<sub>2</sub>I"],"journal":["Nature"],"pubmed_title":["High-resolution in situ structures of mammalian respiratory supercomplexes."],"pmcid":["PMC11222160"],"funding_grant_id":["S10 OD023603","R35 GM142959"],"pubmed_authors":["Zhu J","Zhang K","Chai P","Zheng W"],"additional_accession":[]},"is_claimable":false,"name":"High-resolution in situ structures of mammalian respiratory supercomplexes.","description":"Mitochondria play a pivotal part in ATP energy production through oxidative phosphorylation, which occurs within the inner membrane through a series of respiratory complexes<sup>1-4</sup>. Despite extensive in vitro structural studies, determining the atomic details of their molecular mechanisms in physiological states remains a major challenge, primarily because of loss of the native environment during purification. Here we directly image porcine mitochondria using an in situ cryo-electron microscopy approach. This enables us to determine the structures of various high-order assemblies of respiratory supercomplexes in their native states. We identify four main supercomplex organizations: I<sub>1</sub>III<sub>2</sub>IV<sub>1</sub>, I<sub>1</sub>III<sub>2</sub>IV<sub>2</sub>, I<sub>2</sub>I","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2026-05-29T11:44:38.4Z","creation":"2024-11-14T17:01:26.808Z"},"accession":"S-EPMC11222160","cross_references":{"pubmed":["38811722"],"doi":["10.1038/s41586-024-07488-9"]}}