biostudies-literatureUnknown8(12)Denlinger N<h4>Abstract</h4>Chimeric antigen receptor (CAR) T-cell therapy has revolutionized treatment for relapsed/refractory B-cell non-Hodgkin lymphoma (NHL). Robust biomarkers and a complete understanding of CAR T-cell function in the postinfusion phase remain limited. Here, we used a 37-color spectral flow cytometry panel to perform high dimensional single-cell analysis of postinfusion samples in 26 patients treated with CD28 costimulatory domain containing commercial CAR T cells for NHL and focused on computationally gated CD8+ CAR T cells. We found that the presence of postinfusion Programmed cell death protein 1 (PD-1)+ CD8+ CAR T cells at the day 14 time point highly correlated with the ability to achieve complete response (CR) by 6 months. Further analysis identified multiple subtypes of CBlood advances3140-3153https://www.ebi.ac.uk/biostudies/studies/S-EPMC11222947biostudies-literaturePostinfusion PD-1+ CD8+ CAR T cells identify patients responsive to CD19 CAR T-cell therapy in non-Hodgkin lymphoma.PMC11222947Chan WKJaglowski SBaiocchi RASong NJWu DVoorhees TJMiao JReneau JCReynolds KEpperla NSawalha YRubinstein MPHanel WKittai ASde Lima MAlinari LBond DAWang YDenlinger NSigmund AChristian BMaddocks KPeterson CSong CJeon HVasu SBolz RMBezerra EChung DLi ZYang YZhang XHuang XBrammer JfalsePostinfusion PD-1+ CD8+ CAR T cells identify patients responsive to CD19 CAR T-cell therapy in non-Hodgkin lymphoma.<h4>Abstract</h4>Chimeric antigen receptor (CAR) T-cell therapy has revolutionized treatment for relapsed/refractory B-cell non-Hodgkin lymphoma (NHL). Robust biomarkers and a complete understanding of CAR T-cell function in the postinfusion phase remain limited. Here, we used a 37-color spectral flow cytometry panel to perform high dimensional single-cell analysis of postinfusion samples in 26 patients treated with CD28 costimulatory domain containing commercial CAR T cells for NHL and focused on computationally gated CD8+ CAR T cells. We found that the presence of postinfusion Programmed cell death protein 1 (PD-1)+ CD8+ CAR T cells at the day 14 time point highly correlated with the ability to achieve complete response (CR) by 6 months. Further analysis identified multiple subtypes of C2024-01-01T00:00:00Z2024 Jun2025-04-04T19:23:27.185Z2025-04-04T19:23:27.185ZS-EPMC112229473860738110.1182/bloodadvances.2023012073