biostudies-literatureDelaney SNIAID NIH HHSFoundation for the National Institutes of HealthNCI NIH HHS2547-2557https://www.ebi.ac.uk/biostudies/studies/S-EPMC11223962biostudies-literatureUnknown51(9)<h4>Purpose</h4>Cadherin-17 (CDH17) is a calcium-dependent cell adhesion protein that is overexpressed in several adenocarcinomas, including gastric, colorectal, and pancreatic adenocarcinoma. High levels of CDH17 have been linked to metastatic disease and poor prognoses in patients with these malignancies, fueling interest in the protein as a target for diagnostics and therapeutics. Herein, we report the synthesis, in vitro validation, and in vivo evaluation of a CDH17-targeted ⁸⁹Zr-labeled immunoPET probe.<h4>Methods</h4>The CDH17-targeting mAb D2101 was modified with an isothiocyanate-bearing derivative of desferrioxamine (DFO) to produce a chelator-bearing immunoconjugate - DFO-D2101 - and flow cytometry and surface plasmon resonance (SPR) were used to interrogate its antigeEuropean journal of nuclear medicine and molecular imagingCadherin-17 as a target for the immunoPET of adenocarcinoma.PMC11223962R00CA226363R01 CA2443271R01AI175417P30 CA0087481R01CA2818011R01CA244327R01 CA204167R01CA240963R01 AI175417R00ES034053R01 CA240963R01 CA281801F31CA275334Lam DKeinanen ODelaney SWolfe ALZeglis BMHamakubo TfalseCadherin-17 as a target for the immunoPET of adenocarcinoma.<h4>Purpose</h4>Cadherin-17 (CDH17) is a calcium-dependent cell adhesion protein that is overexpressed in several adenocarcinomas, including gastric, colorectal, and pancreatic adenocarcinoma. High levels of CDH17 have been linked to metastatic disease and poor prognoses in patients with these malignancies, fueling interest in the protein as a target for diagnostics and therapeutics. Herein, we report the synthesis, in vitro validation, and in vivo evaluation of a CDH17-targeted ⁸⁹Zr-labeled immunoPET probe.<h4>Methods</h4>The CDH17-targeting mAb D2101 was modified with an isothiocyanate-bearing derivative of desferrioxamine (DFO) to produce a chelator-bearing immunoconjugate - DFO-D2101 - and flow cytometry and surface plasmon resonance (SPR) were used to interrogate its antige2024-01-01T00:00:00Z2024 Jul2026-06-02T21:18:36.347Z2025-04-04T12:21:36.052ZS-EPMC112239623862540210.1007/s00259-024-06709-7