{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Song Y"],"funding":["the National Key Research and Development Program of China","the Military Logistics Research Project Health Special Project"],"pagination":["15406"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11224322"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Patients with multiple myeloma (MM) experience relapse and drug resistance; therefore, novel treatments are essential. Clotrimazole (CTZ) is a wide-spectrum antifungal drug with antitumor activity. However, CTZ's effects on MM are unclear. We investigated CTZ's effect on MM cell proliferation and apoptosis induction mechanisms. CTZ's effects on MM.1S, NCI- H929, KMS-11, and U266 cell growth were investigated using Cell Counting Kit-8 (CCK-8) assay. The apoptotic cell percentage was quantified with annexin V-fluorescein isothiocyanate/7-amino actinomycin D staining. Mitochondrial membrane potential (MMP) and cell cycle progression were evaluated. Reactive oxygen species (ROS) levels were measured via fluorescence microscopy. Expression of apoptosis-related and nuclear factor (NF)-κB signali"],"journal":["Scientific reports"],"pubmed_title":["Clotrimazole inhibits growth of multiple myeloma cells in vitro via G0/G1 arrest and mitochondrial apoptosis."],"pmcid":["PMC11224322"],"funding_grant_id":["NCRCG-PLAGH-20230010","2021YFC2701704"],"pubmed_authors":["Lu X","Zhang H","Bo Y","Geng J","Chen H","Song Y"],"additional_accession":[]},"is_claimable":false,"name":"Clotrimazole inhibits growth of multiple myeloma cells in vitro via G0/G1 arrest and mitochondrial apoptosis.","description":"Patients with multiple myeloma (MM) experience relapse and drug resistance; therefore, novel treatments are essential. Clotrimazole (CTZ) is a wide-spectrum antifungal drug with antitumor activity. However, CTZ's effects on MM are unclear. We investigated CTZ's effect on MM cell proliferation and apoptosis induction mechanisms. CTZ's effects on MM.1S, NCI- H929, KMS-11, and U266 cell growth were investigated using Cell Counting Kit-8 (CCK-8) assay. The apoptotic cell percentage was quantified with annexin V-fluorescein isothiocyanate/7-amino actinomycin D staining. Mitochondrial membrane potential (MMP) and cell cycle progression were evaluated. Reactive oxygen species (ROS) levels were measured via fluorescence microscopy. Expression of apoptosis-related and nuclear factor (NF)-κB signali","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2025-04-21T16:15:05.743Z","creation":"2025-04-21T16:15:05.743Z"},"accession":"S-EPMC11224322","cross_references":{"pubmed":["38965397"],"doi":["10.1038/s41598-024-66367-5"]}}