{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(1)"],"submitter":["Wang S"],"funding":["funded by Simcere Zaiming"],"pubmed_abstract":["Precise measurement of the binding activity changes of therapeutic antibodies is important to determine the potential critical quality attributes (CQAs) in developability assessment at the early stage of antibody development. Here, we report a surface plasmon resonance (SPR)-based relative binding activity method, which incorporates both binding affinity and binding response and allows us to determine relative binding activity of antibodies with high accuracy and precision. We applied the SPR-based relative binding activity method in multiple forced degradation studies of antibody developability assessment. The current developability assessment strategy provided comprehensive, precise characterization of antibody binding activity in the stability studies, enabling us to perform correlation"],"journal":["mAbs"],"pagination":["2374607"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11225922"],"repository":["biostudies-literature"],"pubmed_title":["Early determination of potential critical quality attributes of therapeutic antibodies in developability studies through surface plasmon resonance-based relative binding activity assessment."],"pmcid":["PMC11225922"],"pubmed_authors":["Fu Y","Chen C","Zhao X","Li Z","Wang Q","Hong Y","Fan J","Wang S","Ge Y","Wang Y","Cheng G","Wang Z","Wu Y"],"additional_accession":[]},"is_claimable":false,"name":"Early determination of potential critical quality attributes of therapeutic antibodies in developability studies through surface plasmon resonance-based relative binding activity assessment.","description":"Precise measurement of the binding activity changes of therapeutic antibodies is important to determine the potential critical quality attributes (CQAs) in developability assessment at the early stage of antibody development. Here, we report a surface plasmon resonance (SPR)-based relative binding activity method, which incorporates both binding affinity and binding response and allows us to determine relative binding activity of antibodies with high accuracy and precision. We applied the SPR-based relative binding activity method in multiple forced degradation studies of antibody developability assessment. The current developability assessment strategy provided comprehensive, precise characterization of antibody binding activity in the stability studies, enabling us to perform correlation","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jan-Dec","modification":"2025-04-04T23:55:35.324Z","creation":"2025-04-04T23:55:35.324Z"},"accession":"S-EPMC11225922","cross_references":{"pubmed":["38956880"],"doi":["10.1080/19420862.2024.2374607"]}}