{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Ji H"],"pubmed_abstract":["<h4>Objective</h4>To study the mechanism by which conditioned medium of bone marrow mesenchymal stem cells (BMSCs-CM) facilitates the transition of pro-inflammatory polarized microglia to an anti-inflammatory phenotype.<h4>Methods</h4>BV2 cells, a mouse microglia cell line, were transformed into a pro-inflammatory phenotype using lipopolysaccharide. The expression of phenotypic genes in BV2 cells was detected using real-time quantitative PCR (RT-qPCR). Enzyme-linked immunosorbent assay was used to measure inflammatory cytokine levels in BV2 cells co-cultured with BMSCs-CM. The expressions of mitophagy-associated proteins were determined using western blot. The mitochondrial membrane potential and ATP levels in BV2 cells were measured using JC-1 staining and an ATP assay kit, respectively. "],"journal":["PeerJ"],"pagination":["e17664"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11227809"],"repository":["biostudies-literature"],"pubmed_title":["Conditioned culture medium of bone marrow mesenchymal stem cells promotes phenotypic transformation of microglia by regulating mitochondrial autophagy."],"pmcid":["PMC11227809"],"pubmed_authors":["Peng X","Yang Y","Ji H","Chu W","Song X"],"additional_accession":[]},"is_claimable":false,"name":"Conditioned culture medium of bone marrow mesenchymal stem cells promotes phenotypic transformation of microglia by regulating mitochondrial autophagy.","description":"<h4>Objective</h4>To study the mechanism by which conditioned medium of bone marrow mesenchymal stem cells (BMSCs-CM) facilitates the transition of pro-inflammatory polarized microglia to an anti-inflammatory phenotype.<h4>Methods</h4>BV2 cells, a mouse microglia cell line, were transformed into a pro-inflammatory phenotype using lipopolysaccharide. The expression of phenotypic genes in BV2 cells was detected using real-time quantitative PCR (RT-qPCR). Enzyme-linked immunosorbent assay was used to measure inflammatory cytokine levels in BV2 cells co-cultured with BMSCs-CM. The expressions of mitophagy-associated proteins were determined using western blot. The mitochondrial membrane potential and ATP levels in BV2 cells were measured using JC-1 staining and an ATP assay kit, respectively. ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024","modification":"2025-04-26T05:42:01.557Z","creation":"2025-04-06T11:36:45.606Z"},"accession":"S-EPMC11227809","cross_references":{"pubmed":["38974415"],"doi":["10.7717/peerj.17664"]}}