biostudies-literatureDing DNational Natural Science Foundation of ChinaSpecial Scientific Research Project of Army Laboratory Animals377-387https://www.ebi.ac.uk/biostudies/studies/S-EPMC11228093biostudies-literatureUnknown7(3)<h4>Background</h4>Atherosclerosis is a chronic cardiovascular disease of great concern. However, it is difficult to establish a direct connection between conventional small animal models and clinical practice. The pig's genome, physiology, and anatomy reflect human biology better than other laboratory animals, which is crucial for studying the pathogenesis of atherosclerosis.<h4>Methods</h4>We used whole-genome sequencing data from nine Bama minipigs to perform a genome-wide linkage analysis, and further used bioinformatic tools to filter and identify underlying candidate genes. Candidate gene function prediction was performed using the online prediction tool STRING 12.0. Immunohistochemistry and immunofluorescence were used to detect the expression of proteins encoded by candidate genes.Animal models and experimental medicineIdentification of novel genes associated with atherosclerosis in Bama miniature pig.PMC1122809332370568SYDW [2020]01Li XZheng XDing DJia YNiu MChen HZhao YfalseIdentification of novel genes associated with atherosclerosis in Bama miniature pig.<h4>Background</h4>Atherosclerosis is a chronic cardiovascular disease of great concern. However, it is difficult to establish a direct connection between conventional small animal models and clinical practice. The pig's genome, physiology, and anatomy reflect human biology better than other laboratory animals, which is crucial for studying the pathogenesis of atherosclerosis.<h4>Methods</h4>We used whole-genome sequencing data from nine Bama minipigs to perform a genome-wide linkage analysis, and further used bioinformatic tools to filter and identify underlying candidate genes. Candidate gene function prediction was performed using the online prediction tool STRING 12.0. Immunohistochemistry and immunofluorescence were used to detect the expression of proteins encoded by candidate genes.2024-01-01T00:00:00Z2024 Jun2025-04-26T05:43:26.052Z2025-04-06T11:35:40.342ZS-EPMC112280933872046910.1002/ame2.12412