{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Fan M"],"funding":["China Scholarship Council","Amsterdam UMC Location AMC","NIAID NIH HHS","National Institutes of Health"],"pagination":["115046"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11228593"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["165"],"pubmed_abstract":["<h4>Background</h4>The novel endonuclease Cas12b was engineered for targeted genome editing in mammalian cells and is a promising tool for certain applications because of its small size, high sequence specificity and ability to generate relatively large deletions. We previously reported inhibition of the human immunodeficiency virus (HIV) in cell culture infections upon attack of the integrated viral DNA genome by spCas9 and Cas12a.<h4>Methods</h4>We now tested the ability of the Cas12b endonuclease to suppress a spreading HIV infection in cell culture with anti-HIV gRNAs. Virus inhibition was tested in long-term HIV replication studies, which allowed us to test for viral escape and the potential for reaching a CURE of the infected T cells.<h4>Findings</h4>We demonstrate that Cas12b can ac"],"journal":["Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie"],"pubmed_title":["CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell cultures."],"pmcid":["PMC11228593"],"funding_grant_id":["1R01AI145045IH","R01 AI145045"],"pubmed_authors":["Bao Y","Fan M","Herrera-Carrillo E","Berkhout B"],"additional_accession":[]},"is_claimable":false,"name":"CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell cultures.","description":"<h4>Background</h4>The novel endonuclease Cas12b was engineered for targeted genome editing in mammalian cells and is a promising tool for certain applications because of its small size, high sequence specificity and ability to generate relatively large deletions. We previously reported inhibition of the human immunodeficiency virus (HIV) in cell culture infections upon attack of the integrated viral DNA genome by spCas9 and Cas12a.<h4>Methods</h4>We now tested the ability of the Cas12b endonuclease to suppress a spreading HIV infection in cell culture with anti-HIV gRNAs. Virus inhibition was tested in long-term HIV replication studies, which allowed us to test for viral escape and the potential for reaching a CURE of the infected T cells.<h4>Findings</h4>We demonstrate that Cas12b can ac","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Sep","modification":"2025-04-26T05:38:16.177Z","creation":"2025-04-06T11:36:01.261Z"},"accession":"S-EPMC11228593","cross_references":{"pubmed":["37379644"],"doi":["10.1016/j.biopha.2023.115046"]}}