biostudies-literatureChakraborty AWellcome Trust390-396https://www.ebi.ac.uk/biostudies/studies/S-EPMC11228930biostudies-literatureUnknown20(6)Glycosyl-inositol-phospho-ceramides (GIPCs) or glycosylphosphatidylinositol-anchored fungal polysaccharides are major lipids in plant and fungal plasma membranes and play an important role in stress adaption. However, their analysis remains challenging due to the multiple steps involved in their extraction and purification prior to mass spectrometry analysis. To address this challenge, we report here a novel simplified method to identify GIPCs from <i>Aspergillus fumigatus</i> using the new Bruker MBT lipid Xtract assay. <i>A. fumigatus</i> reference strains and clinical isolates were cultured, harvested, heat-inactivated and suspended in double-distilled water. A fraction of this fungal preparation was then dried in a microtube, mixed with an MBT lipid Xtract matrix (Bruker Daltonik, GermMolecular omicsIntact cell lipidomics using the Bruker MBT lipid Xtract assay allows the rapid detection of glycosyl-inositol-phospho-ceramides from <i>Aspergillus fumigatus</i>.PMC11228930105603/Z/14/ZAlsharqi LKostrzewa MChakraborty AArmstrong-James DLarrouy-Maumus GfalseIntact cell lipidomics using the Bruker MBT lipid Xtract assay allows the rapid detection of glycosyl-inositol-phospho-ceramides from <i>Aspergillus fumigatus</i>.Glycosyl-inositol-phospho-ceramides (GIPCs) or glycosylphosphatidylinositol-anchored fungal polysaccharides are major lipids in plant and fungal plasma membranes and play an important role in stress adaption. However, their analysis remains challenging due to the multiple steps involved in their extraction and purification prior to mass spectrometry analysis. To address this challenge, we report here a novel simplified method to identify GIPCs from <i>Aspergillus fumigatus</i> using the new Bruker MBT lipid Xtract assay. <i>A. fumigatus</i> reference strains and clinical isolates were cultured, harvested, heat-inactivated and suspended in double-distilled water. A fraction of this fungal preparation was then dried in a microtube, mixed with an MBT lipid Xtract matrix (Bruker Daltonik, Germ2024-01-01T00:00:00Z2024 Jul2025-04-04T12:21:22.136Z2025-04-04T12:21:22.136ZS-EPMC112289303862371110.1039/d4mo00030g