biostudies-literatureSantamaria LAgriculture and Agri-Food Canada14844-14850https://www.ebi.ac.uk/biostudies/studies/S-EPMC11228969biostudies-literatureUnknown72(26)The 11S globulin legumin typically accounts for approximately 3% of the total protein in common beans (<i>Phaseolus vulgaris</i>). It was previously reported that a legumin peptide of approximately 20 kDa is resistant to pepsin digestion. Sequence prediction suggested that the pepsin-resistant peptide is located at the C-terminal end of the α-subunit, within a glutamic acid-rich domain, overlapping with a chymotrypsin-resistant peptide. Using purified legumin, the peptide of approximately 20 kDa was found to be resistant to pepsin digestion in a pH-dependent manner, and its location was determined by two-dimensional gel electrophoresis and LC-MS-MS. The location of the chymotrypsin-resistant peptide was confirmed by immunoblotting with peptide-specific polyclonal antibodies. The presence oJournal of agricultural and food chemistryIdentification and Characterization of a Pepsin- and Chymotrypsin-Resistant Peptide in the α Subunit of the 11S Globulin Legumin from Common Bean (<i>Phaseolus vulgaris</i> L.).PMC11228969J-000562Pajak AHouse JDMarsolais FSantamaria LfalseIdentification and Characterization of a Pepsin- and Chymotrypsin-Resistant Peptide in the α Subunit of the 11S Globulin Legumin from Common Bean (<i>Phaseolus vulgaris</i> L.).The 11S globulin legumin typically accounts for approximately 3% of the total protein in common beans (<i>Phaseolus vulgaris</i>). It was previously reported that a legumin peptide of approximately 20 kDa is resistant to pepsin digestion. Sequence prediction suggested that the pepsin-resistant peptide is located at the C-terminal end of the α-subunit, within a glutamic acid-rich domain, overlapping with a chymotrypsin-resistant peptide. Using purified legumin, the peptide of approximately 20 kDa was found to be resistant to pepsin digestion in a pH-dependent manner, and its location was determined by two-dimensional gel electrophoresis and LC-MS-MS. The location of the chymotrypsin-resistant peptide was confirmed by immunoblotting with peptide-specific polyclonal antibodies. The presence o2024-01-01T00:00:00Z2024 Jul2026-06-01T07:37:43.867Z2025-04-04T11:24:38.376ZS-EPMC112289693888544010.1021/acs.jafc.3c08744