biostudies-literatureWang YNatural Science Foundation of Zhejiang ProvinceState Key Laboratory Foundation for Diagnosis and Treatment of Infectious DiseasesNational Natural Science Foundation of ChinaHangzhou Youth Innovation Team ProjectScientific Research Foundation for Scholars of HZNU7142-7157https://www.ebi.ac.uk/biostudies/studies/S-EPMC11229311biostudies-literatureUnknown52(12)DHH/DHHA1 family proteins have been proposed to play critical roles in bacterial resistance to environmental stresses. Members of the most radioresistant bacteria genus, Deinococcus, possess two DHH/DHHA1 family proteins, RecJ and RecJ-like. While the functions of Deinococcus radiodurans RecJ (DrRecJ) in DNA damage resistance have been well characterized, the role and biochemical activities of D. radiodurans RecJ-like (DrRecJ-like) remain unclear. Phenotypic and transcriptomic analyses suggest that, beyond DNA repair, DrRecJ is implicated in cell growth and division. Additionally, DrRecJ-like not only affects stress response, cell growth, and division but also correlates with the folding/stability of intracellular proteins, as well as the formation and stability of cell membranes/walls. DrNucleic acids researchStructural and functional investigation of the DHH/DHHA1 family proteins in Deinococcus radiodurans.PMC11229311LQ22C0500023220014632270043TD20230204255C50222204016zz2023094125C5022120404032100017Yang JGuo ZCheng KHao WLuo YWang YSun YWu YGao TJin LfalseStructural and functional investigation of the DHH/DHHA1 family proteins in Deinococcus radiodurans.DHH/DHHA1 family proteins have been proposed to play critical roles in bacterial resistance to environmental stresses. Members of the most radioresistant bacteria genus, Deinococcus, possess two DHH/DHHA1 family proteins, RecJ and RecJ-like. While the functions of Deinococcus radiodurans RecJ (DrRecJ) in DNA damage resistance have been well characterized, the role and biochemical activities of D. radiodurans RecJ-like (DrRecJ-like) remain unclear. Phenotypic and transcriptomic analyses suggest that, beyond DNA repair, DrRecJ is implicated in cell growth and division. Additionally, DrRecJ-like not only affects stress response, cell growth, and division but also correlates with the folding/stability of intracellular proteins, as well as the formation and stability of cell membranes/walls. Dr2024-01-01T00:00:00Z2024 Jul2026-04-08T19:22:12.242Z2025-04-04T11:25:34.227ZS-EPMC112293113880426310.1093/nar/gkae451