biostudies-literatureUnknown12Dong J<h4>Background</h4>Oligospermia is one of the most common reasons for male infertility which is troubling numerous couples of child-bearing age. This investigation scrutinizes the implications and mechanistic underpinnings of ursolic acid's effect on busulfan-induced oligospermia in mouse models.<h4>Methods</h4>A singular intraperitoneal injection of busulfan at a dosage of 30 mg/kg induced oligospermia. Two weeks subsequent to this induction, mice were subjected to various dosages of ursolic acid (10, 30, and 50 mg/kg body weight, respectively) on a daily basis for four consecutive weeks. Following this treatment period, a meticulous analysis of epididymal sperm parameters, encompassing concentration and motility, was conducted using a computer-assisted sperm analysis system. The histopatPeerJe17691https://www.ebi.ac.uk/biostudies/studies/S-EPMC11229684biostudies-literatureUrsolic acid attenuates oligospermia in busulfan-induced mice by promoting motor proteins.PMC11229684Dong YDong JYe THui JWang XfalseUrsolic acid attenuates oligospermia in busulfan-induced mice by promoting motor proteins.<h4>Background</h4>Oligospermia is one of the most common reasons for male infertility which is troubling numerous couples of child-bearing age. This investigation scrutinizes the implications and mechanistic underpinnings of ursolic acid's effect on busulfan-induced oligospermia in mouse models.<h4>Methods</h4>A singular intraperitoneal injection of busulfan at a dosage of 30 mg/kg induced oligospermia. Two weeks subsequent to this induction, mice were subjected to various dosages of ursolic acid (10, 30, and 50 mg/kg body weight, respectively) on a daily basis for four consecutive weeks. Following this treatment period, a meticulous analysis of epididymal sperm parameters, encompassing concentration and motility, was conducted using a computer-assisted sperm analysis system. The histopat2024-01-01T00:00:00Z20242026-06-01T07:37:46.152Z2025-04-04T11:24:40.537ZS-EPMC112296843897875210.7717/peerj.17691