{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Nicholas CA"],"funding":["NIDDK NIH HHS","NIA NIH HHS","NCI NIH HHS","NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Autoreactive B cells play an important but ill-defined role in autoimmune type 1 diabetes (T1D). To better understand their contribution, we performed single cell gene and BCR-seq analysis on pancreatic islet antigen-reactive (IAR) B cells from the peripheral blood of nondiabetic (ND), autoantibody positive prediabetic (AAB), and recent-onset T1D individuals. We found that the frequency of IAR B cells was increased in AAB and T1D. IAR B cells from these donors had altered expression of B cell signaling, pro-inflammatory, infection, and antigen processing and presentation genes. Both AAB and T1D donors demonstrated a significant increase in certain heavy and light chain V genes, and these V genes were enriched in islet-reactivity. Public clones of IAR B cells were restricted almost entirely"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.06.20.599914"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11230262"],"repository":["biostudies-literature"],"pubmed_title":["Islet-antigen reactive B cells display a unique phenotype and BCR repertoire in autoantibody positive and recent-onset type 1 diabetes patients."],"pmcid":["PMC11230262"],"funding_grant_id":["P30 CA046934","P30 DK116073","F31 DK134095","T32 GM136444","R01 AG071467","K01 OD028759"],"pubmed_authors":["Evans SA","Wells KL","Smith MJ","Tensun FA","Gottlieb PA","Nicholas CA","Toole KP","Broncucia H","Hesselberth JR"],"additional_accession":[]},"is_claimable":false,"name":"Islet-antigen reactive B cells display a unique phenotype and BCR repertoire in autoantibody positive and recent-onset type 1 diabetes patients.","description":"Autoreactive B cells play an important but ill-defined role in autoimmune type 1 diabetes (T1D). To better understand their contribution, we performed single cell gene and BCR-seq analysis on pancreatic islet antigen-reactive (IAR) B cells from the peripheral blood of nondiabetic (ND), autoantibody positive prediabetic (AAB), and recent-onset T1D individuals. We found that the frequency of IAR B cells was increased in AAB and T1D. IAR B cells from these donors had altered expression of B cell signaling, pro-inflammatory, infection, and antigen processing and presentation genes. Both AAB and T1D donors demonstrated a significant increase in certain heavy and light chain V genes, and these V genes were enriched in islet-reactivity. Public clones of IAR B cells were restricted almost entirely","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2026-06-30T03:17:36.821Z","creation":"2025-04-04T01:54:34.902Z"},"accession":"S-EPMC11230262","cross_references":{"pubmed":["38979376"],"doi":["10.1101/2024.06.20.599914"]}}