{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Prakash D"],"funding":["Advanced Energy Institute, Southern Illinois University-Carbondale","Basic Energy Sciences","National Institute of General Medical Sciences","NIH HHS","NIGMS NIH HHS"],"pagination":["1588-1598"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11234629"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["63(12)"],"pubmed_abstract":["Thioredoxin reductases (TrxR) activate thioredoxins (Trx) that regulate the activity of diverse target proteins essential to prokaryotic and eukaryotic life. However, very little is understood of TrxR/Trx systems and redox control in methanogenic microbes from the domain <i>Archaea</i> (methanogens), for which genomes are abundant with annotations for ferredoxin:thioredoxin reductases [Fdx/thioredoxin reductase (FTR)] from group 4 of the widespread FTR-like family. Only two from the FTR-like family are characterized: the plant-type FTR from group 1 and FDR from group 6. Herein, the group 4 archetype (AFTR) from <i>Methanosarcina acetivorans</i> was characterized to advance understanding of the family and TrxR/Trx systems in methanogens. The modeled structure of AFTR, together with EPR and "],"journal":["Biochemistry"],"pubmed_title":["Catalytic Activity of the Archetype from Group 4 of the FTR-like Ferredoxin:Thioredoxin Reductase Family Is Regulated by Unique <i>S</i> = 7/2 and <i>S</i> = 1/2 [4Fe-4S] Clusters."],"pmcid":["PMC11234629"],"funding_grant_id":["R01 GM125924","DE-FG02-95ER20198","S10 OD028589","DESC0018087","R01-GM125924"],"pubmed_authors":["Xiong J","Guo Y","Prakash D","Chauhan SS","Yennawar N","Golbeck JH","Kruse H","Walters KA","Ferry JG"],"additional_accession":[]},"is_claimable":false,"name":"Catalytic Activity of the Archetype from Group 4 of the FTR-like Ferredoxin:Thioredoxin Reductase Family Is Regulated by Unique <i>S</i> = 7/2 and <i>S</i> = 1/2 [4Fe-4S] Clusters.","description":"Thioredoxin reductases (TrxR) activate thioredoxins (Trx) that regulate the activity of diverse target proteins essential to prokaryotic and eukaryotic life. However, very little is understood of TrxR/Trx systems and redox control in methanogenic microbes from the domain <i>Archaea</i> (methanogens), for which genomes are abundant with annotations for ferredoxin:thioredoxin reductases [Fdx/thioredoxin reductase (FTR)] from group 4 of the widespread FTR-like family. Only two from the FTR-like family are characterized: the plant-type FTR from group 1 and FDR from group 6. Herein, the group 4 archetype (AFTR) from <i>Methanosarcina acetivorans</i> was characterized to advance understanding of the family and TrxR/Trx systems in methanogens. The modeled structure of AFTR, together with EPR and ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2025-04-05T15:48:40.029Z","creation":"2025-04-05T15:48:40.029Z"},"accession":"S-EPMC11234629","cross_references":{"pubmed":["38817151"],"doi":["10.1021/acs.biochem.3c00651"]}}