{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tubero Euzebio Alves V"],"funding":["National Institutes of Health"],"pagination":["2376462"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11234918"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["<h4>Background</h4>Gingipains are important virulence factors present in Porphyromonas gingivalis. Arginine-specific gingipains (RgpA and RgpB) are critically associated with increased proteolytic activity and immune system dysfunction, including neutrophilic activity. In this study, we assessed the impact of gingipains (RgpA and RgpB) on neutrophil function.<h4>Methods</h4>Peripheral blood samples were obtained; neutrophils were isolated and incubated with P. gingivalis A7436, W50, and the double RgpA/RgpB double knockout mutant E8 at MOI 20 for 2 hours. Neutrophil viability was assessed by Sytox staining. Phagocytic capacity and apoptosis were measured by flow cytometry. Superoxide release was measured by superoxide dismutase and cytochrome c reduction assay. Gene expression of TLR2, p47"],"journal":["Journal of oral microbiology"],"pubmed_title":["Arginine-specific gingipains (RgpA/RgpB) knockdown modulates neutrophil machinery."],"pmcid":["PMC11234918"],"funding_grant_id":["AG062496"],"pubmed_authors":["Silva Rovai E","Alves T","Kantarci A","Van Dyke T","Holzhausen M","Tubero Euzebio Alves V","Hasturk H"],"additional_accession":[]},"is_claimable":false,"name":"Arginine-specific gingipains (RgpA/RgpB) knockdown modulates neutrophil machinery.","description":"<h4>Background</h4>Gingipains are important virulence factors present in Porphyromonas gingivalis. Arginine-specific gingipains (RgpA and RgpB) are critically associated with increased proteolytic activity and immune system dysfunction, including neutrophilic activity. In this study, we assessed the impact of gingipains (RgpA and RgpB) on neutrophil function.<h4>Methods</h4>Peripheral blood samples were obtained; neutrophils were isolated and incubated with P. gingivalis A7436, W50, and the double RgpA/RgpB double knockout mutant E8 at MOI 20 for 2 hours. Neutrophil viability was assessed by Sytox staining. Phagocytic capacity and apoptosis were measured by flow cytometry. Superoxide release was measured by superoxide dismutase and cytochrome c reduction assay. Gene expression of TLR2, p47","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024","modification":"2026-06-30T03:10:15.83Z","creation":"2024-11-09T15:15:16.563Z"},"accession":"S-EPMC11234918","cross_references":{"pubmed":["38988325"],"doi":["10.1080/20002297.2024.2376462"]}}