biostudies-literatureUnknown2(1)Shao CThe lung is the respiratory organ of the human body, and the alveoli are the most basic functional units of the lung. Herein, a photo-responsive stretchable Janus membrane was proposed for the reconstruction of the alveolar-capillary barrier in vitro. This Janus membrane was fabricated by photocrosslinking methylacrylamide gelatin (Gelma) hydrogel and N-isoacrylamide (NIPAM) hydrogel mixed with graphene oxide (GO). The Gelma hydrogel containing large amounts of collagen provides a natural extracellular matrix environment for cell growth, while the temperature-sensitive NIPAM hydrogel combined with GO gives the membrane a light-controlled stretching property. Based on this Janus membrane, an open polydimethylsiloxane chip was established to coculture alveolar epithelial cells and vascular eSmart medicinee20220035https://www.ebi.ac.uk/biostudies/studies/S-EPMC11235665biostudies-literatureReconstruction of the alveolar-capillary barrier in vitro based on a photo-responsive stretchable Janus membrane.PMC11235665Cao TWang XYe FFan QShao CfalseReconstruction of the alveolar-capillary barrier in vitro based on a photo-responsive stretchable Janus membrane.The lung is the respiratory organ of the human body, and the alveoli are the most basic functional units of the lung. Herein, a photo-responsive stretchable Janus membrane was proposed for the reconstruction of the alveolar-capillary barrier in vitro. This Janus membrane was fabricated by photocrosslinking methylacrylamide gelatin (Gelma) hydrogel and N-isoacrylamide (NIPAM) hydrogel mixed with graphene oxide (GO). The Gelma hydrogel containing large amounts of collagen provides a natural extracellular matrix environment for cell growth, while the temperature-sensitive NIPAM hydrogel combined with GO gives the membrane a light-controlled stretching property. Based on this Janus membrane, an open polydimethylsiloxane chip was established to coculture alveolar epithelial cells and vascular e2023-01-01T00:00:00Z2023 Feb2025-04-19T19:41:58.812Z2025-04-19T19:41:58.812ZS-EPMC112356653918856310.1002/SMMD.20220035