{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(6)"],"submitter":["Li X"],"pubmed_abstract":["<h4>Aim</h4>To explore the impact of up- or down-regulation of Neurofibromin 2 (NF2) on the expression of downstream Hippo pathway genes, large tumor suppressor gene1 (LATS1), and phosphorylation of Mammalian Ste2-like kinases1/2 (MST1/2), in lung cancer cells.<h4>Methods</h4>A549 lung cancer cells were used. The NF2 was down-regulated by si-RNA interference and upregulated by lentiviral vector mediated overexpression. The LATS1 and MST1/2 expressions were evaluated by real-time PCR and western blot.<h4>Results</h4>Down-regulation of NF2 decreased LATS1 and MST1/2 level (P<0.05). Overexpression of NF2 increased LATS1 (P<0.05) and Mammalian Ste2-like kinases1 (MST1) (P<0.05), suggesting LATS1 and MST1 are modulated by NF2 in a lung cancer cell line.<h4>Conclusions</h4>NF2 mediates the downs"],"journal":["American journal of translational research"],"pagination":["2571-2578"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11236635"],"repository":["biostudies-literature"],"pubmed_title":["Neurofibromin 2 modulates Mammalian Ste2-like kinases1/2 and large tumor suppressor gene1 expression in A549 lung cancer cell line."],"pmcid":["PMC11236635"],"pubmed_authors":["Xiong Y","Li X","Yang Y","Fang J","Guo Z","Qiao S","Zhang X","Ma Y","Wei K"],"additional_accession":[]},"is_claimable":false,"name":"Neurofibromin 2 modulates Mammalian Ste2-like kinases1/2 and large tumor suppressor gene1 expression in A549 lung cancer cell line.","description":"<h4>Aim</h4>To explore the impact of up- or down-regulation of Neurofibromin 2 (NF2) on the expression of downstream Hippo pathway genes, large tumor suppressor gene1 (LATS1), and phosphorylation of Mammalian Ste2-like kinases1/2 (MST1/2), in lung cancer cells.<h4>Methods</h4>A549 lung cancer cells were used. The NF2 was down-regulated by si-RNA interference and upregulated by lentiviral vector mediated overexpression. The LATS1 and MST1/2 expressions were evaluated by real-time PCR and western blot.<h4>Results</h4>Down-regulation of NF2 decreased LATS1 and MST1/2 level (P<0.05). Overexpression of NF2 increased LATS1 (P<0.05) and Mammalian Ste2-like kinases1 (MST1) (P<0.05), suggesting LATS1 and MST1 are modulated by NF2 in a lung cancer cell line.<h4>Conclusions</h4>NF2 mediates the downs","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024","modification":"2026-06-01T13:31:03.477Z","creation":"2025-04-05T15:49:05.396Z"},"accession":"S-EPMC11236635","cross_references":{"pubmed":["39006253"],"doi":["10.62347/TPCM6776","10.62347/tpcm6776"]}}