biostudies-literatureUnknown15Chen YChronic liver disease, a long-term condition resulting from various causes such as alcohol abuse, metabolic disorders, and viral hepatitis, is becoming a significant global health challenge. Gypenosides (GPs), derived from the traditional Chinese medicine <i>Gynostemma pentaphyllum</i> (Thunb.) Makino, exhibited hepatoprotective properties in recent years, yet the precise therapeutic mechanism remains unclear. In this study, label-free and parallel reaction monitoring (PRM) proteomics were used to elucidate the hepatoprotective mechanism of GPs in liver injury rats. Through label-free proteomics, we identified 2104 differentially expressed proteins (DEPs) associated with liver injury, along with 1974 DEPs related to the effects of GPs. Bioinformatics analysis revealed that GPs primarily reFrontiers in pharmacology1417575https://www.ebi.ac.uk/biostudies/studies/S-EPMC11236725biostudies-literatureLabel-free proteomic analysis reveals the hepatoprotective mechanism of gypenosides in liver injury rats.PMC11236725Wang MMa LZhang JChen YWang YPei TfalseLabel-free proteomic analysis reveals the hepatoprotective mechanism of gypenosides in liver injury rats.Chronic liver disease, a long-term condition resulting from various causes such as alcohol abuse, metabolic disorders, and viral hepatitis, is becoming a significant global health challenge. Gypenosides (GPs), derived from the traditional Chinese medicine <i>Gynostemma pentaphyllum</i> (Thunb.) Makino, exhibited hepatoprotective properties in recent years, yet the precise therapeutic mechanism remains unclear. In this study, label-free and parallel reaction monitoring (PRM) proteomics were used to elucidate the hepatoprotective mechanism of GPs in liver injury rats. Through label-free proteomics, we identified 2104 differentially expressed proteins (DEPs) associated with liver injury, along with 1974 DEPs related to the effects of GPs. Bioinformatics analysis revealed that GPs primarily re2024-01-01T00:00:00Z20242026-06-01T07:34:54.887Z2025-04-07T12:03:06.726ZS-EPMC112367253899419910.3389/fphar.2024.1417575