biostudies-literaturePormohammad AEC | European Regional Development Fund (ERDF)Canadian Government | Natural Sciences and Engineering Research Council of Canada (NSERC)EC | European Regional Development FundCanadian Government | Natural Sciences and Engineering Research Council of Canadae0007124https://www.ebi.ac.uk/biostudies/studies/S-EPMC11237694biostudies-literatureUnknown12(6)Novel antimicrobial agents are needed to combat antimicrobial resistance. This study tested novel pentafluorosulfanyl-containing triclocarban analogs for their potential antibacterial efficacy. Standard procedures were used to produce pentafluorosulfanyl-containing triclocarban analogs. Twenty new compounds were tested against seven Gram-positive and Gram-negative indicator strains as well as 10 clinical isolates for their antibacterial and antibiofilm activity. Mechanistic investigations focused on damage to cell membrane, oxidizing reduced thiols, iron-sulfur clusters, and oxidative stress to explain the compounds' activity. Safety profiles were assessed using cytotoxicity experiments in eukaryotic cell lines. Following screening, selected components had significantly better antibacteriaMicrobiology spectrumNovel pentafluorosulfanyl-containing triclocarban analogs selectively kill Gram-positive bacteria.PMC11237694PID2021-122116OB-I00Natural Sciences Engineering Research Council of CanadaCanada Research ChairVazquez SZarei MTurner RJPormohammad APujol ENaesens LHommes JWVazquez-Carrera MSurewaard BGJMoradi MfalseNovel pentafluorosulfanyl-containing triclocarban analogs selectively kill Gram-positive bacteria.Novel antimicrobial agents are needed to combat antimicrobial resistance. This study tested novel pentafluorosulfanyl-containing triclocarban analogs for their potential antibacterial efficacy. Standard procedures were used to produce pentafluorosulfanyl-containing triclocarban analogs. Twenty new compounds were tested against seven Gram-positive and Gram-negative indicator strains as well as 10 clinical isolates for their antibacterial and antibiofilm activity. Mechanistic investigations focused on damage to cell membrane, oxidizing reduced thiols, iron-sulfur clusters, and oxidative stress to explain the compounds' activity. Safety profiles were assessed using cytotoxicity experiments in eukaryotic cell lines. Following screening, selected components had significantly better antibacteria2024-01-01T00:00:00Z2024 Jun2025-04-19T20:07:31.11Z2025-04-19T20:07:31.11ZS-EPMC112376943870032110.1128/spectrum.00071-24