{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hoffmann A"],"funding":["Deutsche Forschungsgemeinschaft (DFG)","Bundesministerium für Bildung und Forschung","Deutsche Forschungsgemeinschaft","Jürgen Manchot Stiftung","Open Access Publication Fund of the University of Bonn","Jürgen Manchot Stiftung (Jürgen Manchot Foundation)","Bundesministerium für Bildung und Forschung (BMBF)"],"pagination":["e0076423"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11237698"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(5)"],"pubmed_abstract":["Cervimycins A-D are bis-glycosylated polyketide antibiotics produced by <i>Streptomyces tendae</i> HKI 0179 with bactericidal activity against Gram-positive bacteria. In this study, cervimycin C (CmC) treatment caused a spaghetti-like phenotype in <i>Bacillus subtilis</i> 168, with elongated curved cells, which stayed joined after cell division, and exhibited a chromosome segregation defect, resulting in ghost cells without DNA. Electron microscopy of CmC-treated <i>Staphylococcus aureus</i> (3 × MIC) revealed swollen cells, misshapen septa, cell wall thickening, and a rough cell wall surface. Incorporation tests in <i>B. subtilis</i> indicated an effect on DNA biosynthesis at high cervimycin concentrations. Indeed, artificial downregulation of the DNA gyrase subunit B gene (<i>gyrB</i>) i"],"journal":["mSphere"],"pubmed_title":["The unusual mode of action of the polyketide glycoside antibiotic cervimycin C."],"pmcid":["PMC11237698"],"funding_grant_id":["398967434","03ZZ0808D"],"pubmed_authors":["Sahl H-G","Bierbaum G","Nieselt K","Hertweck C","Franz-Wachtel M","Scherlach K","Hoffmann A","Steffens U","Macek B","Harbig TA"],"additional_accession":[]},"is_claimable":false,"name":"The unusual mode of action of the polyketide glycoside antibiotic cervimycin C.","description":"Cervimycins A-D are bis-glycosylated polyketide antibiotics produced by <i>Streptomyces tendae</i> HKI 0179 with bactericidal activity against Gram-positive bacteria. In this study, cervimycin C (CmC) treatment caused a spaghetti-like phenotype in <i>Bacillus subtilis</i> 168, with elongated curved cells, which stayed joined after cell division, and exhibited a chromosome segregation defect, resulting in ghost cells without DNA. Electron microscopy of CmC-treated <i>Staphylococcus aureus</i> (3 × MIC) revealed swollen cells, misshapen septa, cell wall thickening, and a rough cell wall surface. Incorporation tests in <i>B. subtilis</i> indicated an effect on DNA biosynthesis at high cervimycin concentrations. Indeed, artificial downregulation of the DNA gyrase subunit B gene (<i>gyrB</i>) i","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-04-29T12:08:33.841Z","creation":"2025-04-07T12:00:08.369Z"},"accession":"S-EPMC11237698","cross_references":{"pubmed":["38722162"],"doi":["10.1128/msphere.00764-23"]}}