{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hume AJ"],"funding":["HHS | National Institutes of Health","HHS | National Institutes of Health (NIH)","NIAID NIH HHS","NIGMS NIH HHS"],"pagination":["e0098424"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11237716"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(6)"],"pubmed_abstract":["<u>In</u>tervening pro<u>teins</u> (inteins) are translated as subdomains within host proteins and removed through an intein-driven splicing reaction where the flanking sequences (exteins) are joined with a peptide bond. Previously, we developed a self-removing translation reporter for labeling Ebola virus (EBOV). In this reporter, an intein (RadA) containing the fluorescent protein ZsGreen (ZsG) is inserted within the EBOV protein VP30. Upon VP30-RadA-ZsG expression from the viral genome, RadA-ZsG is removed from VP30 through the protein splicing activity of RadA, generating functional, non-tagged VP30 and functional ZsGreen. While incorporation of our VP30-RadA-ZsG fusion reporter into recombinant EBOV (rEBOV-RadA-ZsG) resulted in an infectious virus that expresses ZsG upon infection of "],"journal":["mBio"],"pubmed_title":["Improved protein splicing through viral passaging."],"pmcid":["PMC11237716"],"funding_grant_id":["R01AI133486, R21AI169646","R15GM143662, P20GM103436","P20 GM103436","R15 GM143662","R21 AI169646","R01 AI133486"],"pubmed_authors":["Connor JH","Hume AJ","Smetana JS","Muhlberger E","Lennon CW","Turcinovic J","Deeney DJ","Belfort M"],"additional_accession":[]},"is_claimable":false,"name":"Improved protein splicing through viral passaging.","description":"<u>In</u>tervening pro<u>teins</u> (inteins) are translated as subdomains within host proteins and removed through an intein-driven splicing reaction where the flanking sequences (exteins) are joined with a peptide bond. Previously, we developed a self-removing translation reporter for labeling Ebola virus (EBOV). In this reporter, an intein (RadA) containing the fluorescent protein ZsGreen (ZsG) is inserted within the EBOV protein VP30. Upon VP30-RadA-ZsG expression from the viral genome, RadA-ZsG is removed from VP30 through the protein splicing activity of RadA, generating functional, non-tagged VP30 and functional ZsGreen. While incorporation of our VP30-RadA-ZsG fusion reporter into recombinant EBOV (rEBOV-RadA-ZsG) resulted in an infectious virus that expresses ZsG upon infection of ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2026-04-29T12:08:28.648Z","creation":"2025-04-07T12:04:12.752Z"},"accession":"S-EPMC11237716","cross_references":{"pubmed":["38780266"],"doi":["10.1128/mbio.00984-24"]}}