{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["43(1)"],"submitter":["Luo W"],"pubmed_abstract":["<h4>Background</h4>Macrophages play important roles in phagocytosing tumor cells. However, tumors escape macrophage phagocytosis in part through the expression of anti-phagocytic signals, most commonly CD47. In Ewing sarcoma (ES), we found that tumor cells utilize dual mechanisms to evade macrophage clearance by simultaneously over-expressing CD47 and down-regulating cell surface calreticulin (csCRT), the pro-phagocytic signal. Here, we investigate the combination of a CD47 blockade (magrolimab, MAG) to inhibit the anti-phagocytic signal and a chemotherapy regimen (doxorubicin, DOX) to enhance the pro-phagocytic signal to induce macrophage phagocytosis of ES cells in vitro and inhibit tumor growth and metastasis in vivo.<h4>Methods</h4>Macrophages were derived from human peripheral blood m"],"journal":["Journal of experimental & clinical cancer research : CR"],"pagination":["193"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11238356"],"repository":["biostudies-literature"],"pubmed_title":["Combinatorial macrophage induced innate immunotherapy against Ewing sarcoma: Turning \"Two Keys\" simultaneously."],"pmcid":["PMC11238356"],"pubmed_authors":["Liao Y","Rosenblum JM","Costello H","Wijeratne S","Cassady KA","Zhu H","Hoang H","Cairo MS","Ayello J","Miller KE","Chemnitz W","Mardis ER","Gandhi R","Cripe TP","Luo W","Xu S","Gardenswartz A","Lee DA","Mo X","Garfinkle EAR"],"additional_accession":[]},"is_claimable":false,"name":"Combinatorial macrophage induced innate immunotherapy against Ewing sarcoma: Turning \"Two Keys\" simultaneously.","description":"<h4>Background</h4>Macrophages play important roles in phagocytosing tumor cells. However, tumors escape macrophage phagocytosis in part through the expression of anti-phagocytic signals, most commonly CD47. In Ewing sarcoma (ES), we found that tumor cells utilize dual mechanisms to evade macrophage clearance by simultaneously over-expressing CD47 and down-regulating cell surface calreticulin (csCRT), the pro-phagocytic signal. Here, we investigate the combination of a CD47 blockade (magrolimab, MAG) to inhibit the anti-phagocytic signal and a chemotherapy regimen (doxorubicin, DOX) to enhance the pro-phagocytic signal to induce macrophage phagocytosis of ES cells in vitro and inhibit tumor growth and metastasis in vivo.<h4>Methods</h4>Macrophages were derived from human peripheral blood m","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2026-06-01T13:31:38.639Z","creation":"2026-04-08T13:10:42.91Z"},"accession":"S-EPMC11238356","cross_references":{"pubmed":["38992659"],"doi":["10.1186/s13046-024-03093-w"]}}