biostudies-literatureZhang XKYunnan Fundamental Research ProjectsInternational (Foreign) Science and Technology Cooperation Base of Kunming CityYunnan Youth Talent Program- "Ten Thousand Plan"National Natural Science Foundation of ChinaYunnan Provincial Science and Technology Department- Applied Basic Research Joint Special Funds of Chinese MedicineYunnan Province Education Department Research Fund Project3235https://www.ebi.ac.uk/biostudies/studies/S-EPMC11243182biostudies-literatureUnknown29(13)<i>Panax notoginseng</i> is a highly valued perennial medicinal herb in China and is widely used in clinical treatments. The main purpose of this study was to elucidate the changes in the composition of <i>P. notoginseng</i> saponins (PNSs), which are the main bioactive substances, triggered by arbuscular mycorrhizal fungi (AMF) via ultrahigh-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS). A total of 202 putative terpenoid metabolites were detected, of which 150 triterpene glycosides were identified, accounting for 74.26% of the total. Correlation analysis, principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) of the metabolites revealed that the samples treated with AMF (group Ce) could Molecules (Basel, Switzerland)Widely Targeted Metabolomic Analysis Reveals the Improvement in <i>Panax notoginseng</i> Triterpenoids Triggered by Arbuscular Mycorrhizal Fungi via UPLC-ESI-MS/MS.PMC11243182YNWR-QNBJ-2020-279GHJD-20210302023Y0450202101AZ070001-014, 202001AZ070001-00982260743, 82360750202201AT070219You XXZhang XKHe SLong XNWu YCao GHChen DBi YfalseWidely Targeted Metabolomic Analysis Reveals the Improvement in <i>Panax notoginseng</i> Triterpenoids Triggered by Arbuscular Mycorrhizal Fungi via UPLC-ESI-MS/MS.<i>Panax notoginseng</i> is a highly valued perennial medicinal herb in China and is widely used in clinical treatments. The main purpose of this study was to elucidate the changes in the composition of <i>P. notoginseng</i> saponins (PNSs), which are the main bioactive substances, triggered by arbuscular mycorrhizal fungi (AMF) via ultrahigh-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS). A total of 202 putative terpenoid metabolites were detected, of which 150 triterpene glycosides were identified, accounting for 74.26% of the total. Correlation analysis, principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) of the metabolites revealed that the samples treated with AMF (group Ce) could 2024-01-01T00:00:00Z2024 Jul2026-07-09T11:12:59.261Z2026-07-09T10:43:13.374ZS-EPMC112431823899918610.3390/molecules29133235