biostudies-literatureWai MHThe China Postdoc-toral Science Foundation (2022MD713731) to LW, the Science and Technology Major Pro-ject of Guangxi (Gui Ke AA22068096)1850https://www.ebi.ac.uk/biostudies/studies/S-EPMC11243833biostudies-literatureUnknown13(13)Pineapple is a globally significant tropical fruit, but its cultivation faces numerous challenges due to abiotic and biotic stresses, affecting its quality and quantity. WRKY transcription factors are known regulators of stress responses, however, their specific functions in pineapple are not fully understood. This study investigates the role of <i>AcWRKY31</i> by overexpressing it in pineapple and <i>Arabidopsis</i>. Transgenic pineapple lines were obtained using <i>Agrobacterium</i>-mediated transformation methods and abiotic and biotic stress treatments. Transgenic <i>AcWRKY31-OE</i> pineapple plants showed an increased sensitivity to salt and drought stress and an increased resistance to biotic stress from pineapple mealybugs compared to that of WT plants. Similar experiments in <i>AcWPlants (Basel, Switzerland)Overexpression of <i>AcWRKY31</i> Increases Sensitivity to Salt and Drought and Improves Tolerance to Mealybugs in Pineapple.PMC11243833(2022MD713731) and (Gui Ke AA22068096)Wai MHAslam MHuang DZhou QQin YPriyadarshani SVGNCai HChai GWang LLiu YMohammadi MALiu CWang XCheng HLuo TfalseOverexpression of <i>AcWRKY31</i> Increases Sensitivity to Salt and Drought and Improves Tolerance to Mealybugs in Pineapple.Pineapple is a globally significant tropical fruit, but its cultivation faces numerous challenges due to abiotic and biotic stresses, affecting its quality and quantity. WRKY transcription factors are known regulators of stress responses, however, their specific functions in pineapple are not fully understood. This study investigates the role of <i>AcWRKY31</i> by overexpressing it in pineapple and <i>Arabidopsis</i>. Transgenic pineapple lines were obtained using <i>Agrobacterium</i>-mediated transformation methods and abiotic and biotic stress treatments. Transgenic <i>AcWRKY31-OE</i> pineapple plants showed an increased sensitivity to salt and drought stress and an increased resistance to biotic stress from pineapple mealybugs compared to that of WT plants. Similar experiments in <i>AcW2024-01-01T00:00:00Z2024 Jul2025-04-19T12:37:15.871Z2025-04-19T12:37:15.871ZS-EPMC112438333899969010.3390/plants13131850