biostudies-literatureWang STingbo,JiangNational Natural Science Foundation of China1835https://www.ebi.ac.uk/biostudies/studies/S-EPMC11244237biostudies-literatureUnknown13(13)The bHLH transcription factor family plays crucial roles in plant growth and development and their responses to adversity. In this study, a highly salt-induced bHLH gene, <i>PagbHLH35</i> (Potri.018G141600), was identified from <i>Populus alba × P. glandullosa</i> (84K poplar). PagbHLH35 contains a highly conserved bHLH domain within the region of 52-114 amino acids. A subcellular localization result confirmed its nuclear localization. A yeast two-hybrid assay indicated PagbHLH35 lacks transcriptional activation activity, while a yeast one-hybrid assay indicated it could specifically bind to G-box and E-box elements. The expression of <i>PagbHLH35</i> reached its peak at 12 h and 36 h time points under salt stress in the leaves and roots, respectively. A total of three positive transgenic Plants (Basel, Switzerland)<i>PagbHLH35</i> Enhances Salt Tolerance through Improving ROS Scavenging in Transgenic Poplar.PMC1124423731570659Wang SDong LJiang TYao Wfalse<i>PagbHLH35</i> Enhances Salt Tolerance through Improving ROS Scavenging in Transgenic Poplar.The bHLH transcription factor family plays crucial roles in plant growth and development and their responses to adversity. In this study, a highly salt-induced bHLH gene, <i>PagbHLH35</i> (Potri.018G141600), was identified from <i>Populus alba × P. glandullosa</i> (84K poplar). PagbHLH35 contains a highly conserved bHLH domain within the region of 52-114 amino acids. A subcellular localization result confirmed its nuclear localization. A yeast two-hybrid assay indicated PagbHLH35 lacks transcriptional activation activity, while a yeast one-hybrid assay indicated it could specifically bind to G-box and E-box elements. The expression of <i>PagbHLH35</i> reached its peak at 12 h and 36 h time points under salt stress in the leaves and roots, respectively. A total of three positive transgenic 2024-01-01T00:00:00Z2024 Jul2026-07-09T11:05:37.943Z2025-04-19T12:39:01.469ZS-EPMC112442373899967510.3390/plants13131835