{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Medina-Feliciano JG"],"funding":["NIGMS NIH HHS"],"pubmed_abstract":["In holothurians, the regenerative process following evisceration involves the development of a \"rudiment\" or \"anlage\" at the injured end of the mesentery. This regenerating anlage plays a pivotal role in the formation of a new intestine. Despite its significance, our understanding of the molecular characteristics inherent to the constituent cells of this structure has remained limited. To address this gap, we employed state-of-the-art scRNA-seq and HCR-FISH analyses to discern the distinct cellular populations associated with the regeneration anlage. Through this approach, we successfully identified thirteen distinct cell clusters. Among these, two clusters exhibit characteristics consistent with putative mesenchymal cells, while another four show features akin to coelomocyte cell populati"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.07.01.601561"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11244903"],"repository":["biostudies-literature"],"pubmed_title":["Single-cell RNA sequencing of the holothurian regenerating intestine reveals the pluripotency of the coelomic epithelium."],"pmcid":["PMC11244903"],"funding_grant_id":["R25 GM061151","R15 GM124595","P20 GM103475"],"pubmed_authors":["Medina-Feliciano JG","Valentin-Tirado G","Garcia-Arraras JE","Luna-Martinez K","Beltran-Rivera A","Miranda-Negron Y"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell RNA sequencing of the holothurian regenerating intestine reveals the pluripotency of the coelomic epithelium.","description":"In holothurians, the regenerative process following evisceration involves the development of a \"rudiment\" or \"anlage\" at the injured end of the mesentery. This regenerating anlage plays a pivotal role in the formation of a new intestine. Despite its significance, our understanding of the molecular characteristics inherent to the constituent cells of this structure has remained limited. To address this gap, we employed state-of-the-art scRNA-seq and HCR-FISH analyses to discern the distinct cellular populations associated with the regeneration anlage. Through this approach, we successfully identified thirteen distinct cell clusters. Among these, two clusters exhibit characteristics consistent with putative mesenchymal cells, while another four show features akin to coelomocyte cell populati","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2025-04-04T14:46:42.37Z","creation":"2025-04-04T14:46:42.37Z"},"accession":"S-EPMC11244903","cross_references":{"pubmed":["39005414"],"doi":["10.1101/2024.07.01.601561"]}}