{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Rios-Delgado G"],"funding":["NIEHS NIH HHS","NIAID NIH HHS"],"pubmed_abstract":["<i>Staphylococcus aureus</i> has evolved mechanisms to cope with low iron (Fe) availability in host tissues. <i>S. aureus</i> uses the ferric uptake transcriptional regulator (Fur) to sense titers of cytosolic Fe. Upon Fe depletion, apo-Fur relieves transcriptional repression of genes utilized for Fe uptake. We demonstrate that an <i>S. aureus</i> Δ<i>fur</i> mutant has decreased expression of <i>acnA</i>, which codes for the Fe-dependent enzyme aconitase. Decreased <i>acnA</i> expression prevented the Δ<i>fur</i> mutant from growing with amino acids as sole carbon and energy sources. Suppressor analysis determined that a mutation in <i>isrR</i>, which produces a regulatory RNA, permitted growth by decreasing <i>isrR</i> transcription. The decreased AcnA activity of the Δ<i>fur</i> mutant "],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.07.03.601953"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11245030"],"repository":["biostudies-literature"],"pubmed_title":["The &lt;i&gt;Staphylococcus aureus&lt;/i&gt; small non-coding RNA IsrR regulates TCA cycle activity and virulence."],"pmcid":["PMC11245030"],"funding_grant_id":["R21 AI156251","R21 AI153646","T32 ES007028","F31 AI172352"],"pubmed_authors":["Lalaouna D","Zelzion E","Rios-Delgado G","Briaud P","Norambuena J","McReynolds AKG","Kim J","Bose JL","Zheng V","Parker D","Pagella EA","Racine H","Boyd JM","Carroll R","Munneke MJ","Skaar E"],"additional_accession":[]},"is_claimable":false,"name":"The &lt;i&gt;Staphylococcus aureus&lt;/i&gt; small non-coding RNA IsrR regulates TCA cycle activity and virulence.","description":"<i>Staphylococcus aureus</i> has evolved mechanisms to cope with low iron (Fe) availability in host tissues. <i>S. aureus</i> uses the ferric uptake transcriptional regulator (Fur) to sense titers of cytosolic Fe. Upon Fe depletion, apo-Fur relieves transcriptional repression of genes utilized for Fe uptake. We demonstrate that an <i>S. aureus</i> Δ<i>fur</i> mutant has decreased expression of <i>acnA</i>, which codes for the Fe-dependent enzyme aconitase. Decreased <i>acnA</i> expression prevented the Δ<i>fur</i> mutant from growing with amino acids as sole carbon and energy sources. Suppressor analysis determined that a mutation in <i>isrR</i>, which produces a regulatory RNA, permitted growth by decreasing <i>isrR</i> transcription. The decreased AcnA activity of the Δ<i>fur</i> mutant ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2025-04-04T01:18:52.549Z","creation":"2025-04-04T01:18:52.549Z"},"accession":"S-EPMC11245030","cross_references":{"pubmed":["39005296"],"doi":["10.1101/2024.07.03.601953"]}}