biostudies-literatureUnknownRios-Delgado GNIEHS NIH HHSNIAID NIH HHS<i>Staphylococcus aureus</i> has evolved mechanisms to cope with low iron (Fe) availability in host tissues. <i>S. aureus</i> uses the ferric uptake transcriptional regulator (Fur) to sense titers of cytosolic Fe. Upon Fe depletion, apo-Fur relieves transcriptional repression of genes utilized for Fe uptake. We demonstrate that an <i>S. aureus</i> Δ<i>fur</i> mutant has decreased expression of <i>acnA</i>, which codes for the Fe-dependent enzyme aconitase. Decreased <i>acnA</i> expression prevented the Δ<i>fur</i> mutant from growing with amino acids as sole carbon and energy sources. Suppressor analysis determined that a mutation in <i>isrR</i>, which produces a regulatory RNA, permitted growth by decreasing <i>isrR</i> transcription. The decreased AcnA activity of the Δ<i>fur</i> mutant bioRxiv : the preprint server for biology2024.07.03.601953https://www.ebi.ac.uk/biostudies/studies/S-EPMC11245030biostudies-literatureThe <i>Staphylococcus aureus</i> small non-coding RNA IsrR regulates TCA cycle activity and virulence.PMC11245030R21 AI156251R21 AI153646T32 ES007028F31 AI172352Lalaouna DZelzion ERios-Delgado GBriaud PNorambuena JMcReynolds AKGKim JBose JLZheng VParker DPagella EARacine HBoyd JMCarroll RMunneke MJSkaar EfalseThe <i>Staphylococcus aureus</i> small non-coding RNA IsrR regulates TCA cycle activity and virulence.<i>Staphylococcus aureus</i> has evolved mechanisms to cope with low iron (Fe) availability in host tissues. <i>S. aureus</i> uses the ferric uptake transcriptional regulator (Fur) to sense titers of cytosolic Fe. Upon Fe depletion, apo-Fur relieves transcriptional repression of genes utilized for Fe uptake. We demonstrate that an <i>S. aureus</i> Δ<i>fur</i> mutant has decreased expression of <i>acnA</i>, which codes for the Fe-dependent enzyme aconitase. Decreased <i>acnA</i> expression prevented the Δ<i>fur</i> mutant from growing with amino acids as sole carbon and energy sources. Suppressor analysis determined that a mutation in <i>isrR</i>, which produces a regulatory RNA, permitted growth by decreasing <i>isrR</i> transcription. The decreased AcnA activity of the Δ<i>fur</i> mutant 2024-01-01T00:00:00Z2024 Jul2025-04-04T01:18:52.549Z2025-04-04T01:18:52.549ZS-EPMC112450303900529610.1101/2024.07.03.601953