biostudies-literatureUnknown13(6)Li X<h4>Background</h4>There have been studies on the role of sperm-associated antigen 6 <i>(SPAG6</i>) in cytoskeleton formation and growth cone stability, but it is also unknown how spag6 affect tumor growth and development. The aim of this study was to clarify the role of <i>SPAG6</i> in pan-cancer, with some findings about thyroid carcinoma (THCA) validated through experiments.<h4>Methods</h4>We examined the role of <i>SPAG6</i> in pan-cancer, with the data being collected from databases. Further analysis was conducted to assess its correlations with prognosis, gene heterogeneity, stemness, and tumor immunity. The interacting proteins of <i>SPAG6</i> were also identified, and gene ontology enrichment analysis was performed to determine its biological function. We preliminarily confirmed thGland surgery999-1015https://www.ebi.ac.uk/biostudies/studies/S-EPMC11247597biostudies-literatureA comprehensive pan-cancer analysis revealing <i>SPAG6</i> as a novel diagnostic, prognostic and immunological biomarker in tumor.PMC11247597Li XKong LZhang DWang HWang YDiez JJfalseA comprehensive pan-cancer analysis revealing <i>SPAG6</i> as a novel diagnostic, prognostic and immunological biomarker in tumor.<h4>Background</h4>There have been studies on the role of sperm-associated antigen 6 <i>(SPAG6</i>) in cytoskeleton formation and growth cone stability, but it is also unknown how spag6 affect tumor growth and development. The aim of this study was to clarify the role of <i>SPAG6</i> in pan-cancer, with some findings about thyroid carcinoma (THCA) validated through experiments.<h4>Methods</h4>We examined the role of <i>SPAG6</i> in pan-cancer, with the data being collected from databases. Further analysis was conducted to assess its correlations with prognosis, gene heterogeneity, stemness, and tumor immunity. The interacting proteins of <i>SPAG6</i> were also identified, and gene ontology enrichment analysis was performed to determine its biological function. We preliminarily confirmed th2024-01-01T00:00:00Z2024 Jun2025-04-26T05:35:30.741Z2025-04-06T11:30:24.956ZS-EPMC112475973901570510.21037/gs-24-157