biostudies-literatureYu QGuangzhou Science and Technology Plan Projectthe Guangzhou Science and Technology Plan ProjectGuangzhou Municipal Institute Enterprise Joint Funding ProjectScience and Technology Project of FoshanNatural Science Foundation of Guangdong Province50https://www.ebi.ac.uk/biostudies/studies/S-EPMC11247778biostudies-literatureUnknown21(1)<h4>Background</h4>Estrogen receptor-positive (ER+) breast cancer accounts for two-thirds of all breast cancers, and its early and late recurrences still threaten patients' long-term survival and quality of life. Finding candidate tumor antigens and potential therapeutic targets is critical to addressing these unmet needs.<h4>Method</h4>The isobaric tags for relative and absolute quantitation (iTRAQ) proteomic analysis was employed to identify the differentially expressed proteins (DEPs) between ER + breast cancer and corresponding adjacent normal tissue. Candidate DEPs were screened by bioinformatic analyses, and their expression was confirmed by immunohistochemical (IHC) staining and western blot. A series of in vitro experiments, including wound healing assay, colony formation, and cellClinical proteomicsESF1 and MIPEP proteins promote estrogen receptor-positive breast cancer proliferation and are associated with patient prognosis.PMC112477782021030000512021020101012220001004797SL2024A03J003122024A04J99042019A1515110044Qu CYang ZChen PLi ZZhang KZhang YYu QLiang JLiu SYang ASun HfalseESF1 and MIPEP proteins promote estrogen receptor-positive breast cancer proliferation and are associated with patient prognosis.<h4>Background</h4>Estrogen receptor-positive (ER+) breast cancer accounts for two-thirds of all breast cancers, and its early and late recurrences still threaten patients' long-term survival and quality of life. Finding candidate tumor antigens and potential therapeutic targets is critical to addressing these unmet needs.<h4>Method</h4>The isobaric tags for relative and absolute quantitation (iTRAQ) proteomic analysis was employed to identify the differentially expressed proteins (DEPs) between ER + breast cancer and corresponding adjacent normal tissue. Candidate DEPs were screened by bioinformatic analyses, and their expression was confirmed by immunohistochemical (IHC) staining and western blot. A series of in vitro experiments, including wound healing assay, colony formation, and cell2024-01-01T00:00:00Z2024 Jul2025-04-25T19:12:54.27Z2025-04-06T07:53:50.384ZS-EPMC112477783900471710.1186/s12014-024-09502-8