{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Brodie NI"],"funding":["Natural Sciences and Engineering Research Council of Canada","NCI NIH HHS","Canarie"],"pagination":["2146-2155"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11250984"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["34(10)"],"pubmed_abstract":["Crosslinking mass spectrometry (XL-MS) supports structure analysis of individual proteins and highly complex whole-cell interactomes. The identification of crosslinked peptides from enzymatic digests remains challenging, especially at the cell level. Empirical methods that use gas-phase cleavable crosslinkers can simplify the identification process by enabling an MS<sup>3</sup>-based strategy that turns crosslink identification into a simpler problem of detecting two separable peptides. However, the method is limited to select instrument platforms and is challenged by duty cycle constraints. Here, we revisit a pseudo-MS<sup>3</sup> concept that incorporates in-source fragmentation, where a fast switch between gentle high-transmission source conditions and harsher in-source fragmentation se"],"journal":["Journal of the American Society for Mass Spectrometry"],"pubmed_title":["All-in-One Pseudo-MS<sup>3</sup> Method for the Analysis of Gas-Phase Cleavable Protein Crosslinking Reactions."],"pmcid":["PMC11250984"],"funding_grant_id":["P01 CA092584","RGPIN-2017-04879","RS-326"],"pubmed_authors":["Crowder DA","Brodie NI","Sarpe V","Schriemer D"],"additional_accession":[]},"is_claimable":false,"name":"All-in-One Pseudo-MS<sup>3</sup> Method for the Analysis of Gas-Phase Cleavable Protein Crosslinking Reactions.","description":"Crosslinking mass spectrometry (XL-MS) supports structure analysis of individual proteins and highly complex whole-cell interactomes. The identification of crosslinked peptides from enzymatic digests remains challenging, especially at the cell level. Empirical methods that use gas-phase cleavable crosslinkers can simplify the identification process by enabling an MS<sup>3</sup>-based strategy that turns crosslink identification into a simpler problem of detecting two separable peptides. However, the method is limited to select instrument platforms and is challenged by duty cycle constraints. Here, we revisit a pseudo-MS<sup>3</sup> concept that incorporates in-source fragmentation, where a fast switch between gentle high-transmission source conditions and harsher in-source fragmentation se","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Oct","modification":"2025-06-01T00:12:05.24Z","creation":"2024-11-12T23:23:11.037Z"},"accession":"S-EPMC11250984","cross_references":{"pubmed":["37590165"],"doi":["10.1021/jasms.3c00134"]}}