{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14(1)"],"submitter":["Kondo A"],"funding":["MIU Foundation"],"pubmed_abstract":["Presepsin (P-SEP) is a specific biomarker for sepsis. Monocytes produce P-SEP by phagocytosing neutrophil extracellular traps (NETs). Herein, we investigated whether M1 macrophages (M1 MΦs) are the primary producers of P-SEP after NET phagocytosis. We co-cultured M1 MΦs and NETs from healthy participants, measured P-SEP levels in the culture medium supernatant, and detected P-SEP using western blotting. When NETs were co-cultured with M1 MΦs, the P-SEP level of the culture supernatant was high. Notably, we demonstrated, for the first time, the intracellular kinetics of P-SEP production by M1 MΦs via NET phagocytosis: M1 MΦs produced P-SEP intracellularly 15 min after NET phagocytosis and then released it extracellularly. In a sepsis mouse model, the blood NET ratio and P-SEP levels, detect"],"journal":["Scientific reports"],"pagination":["16386"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11252129"],"repository":["biostudies-literature"],"pubmed_title":["Identification of organs of origin of macrophages that produce presepsin via neutrophil extracellular trap phagocytosis."],"pmcid":["PMC11252129"],"pubmed_authors":["Morinishi T","Ikegame A","Yamaguchi Y","Kondo A"],"additional_accession":[]},"is_claimable":false,"name":"Identification of organs of origin of macrophages that produce presepsin via neutrophil extracellular trap phagocytosis.","description":"Presepsin (P-SEP) is a specific biomarker for sepsis. Monocytes produce P-SEP by phagocytosing neutrophil extracellular traps (NETs). Herein, we investigated whether M1 macrophages (M1 MΦs) are the primary producers of P-SEP after NET phagocytosis. We co-cultured M1 MΦs and NETs from healthy participants, measured P-SEP levels in the culture medium supernatant, and detected P-SEP using western blotting. When NETs were co-cultured with M1 MΦs, the P-SEP level of the culture supernatant was high. Notably, we demonstrated, for the first time, the intracellular kinetics of P-SEP production by M1 MΦs via NET phagocytosis: M1 MΦs produced P-SEP intracellularly 15 min after NET phagocytosis and then released it extracellularly. In a sepsis mouse model, the blood NET ratio and P-SEP levels, detect","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2025-04-26T08:04:24.031Z","creation":"2025-04-06T12:35:08.496Z"},"accession":"S-EPMC11252129","cross_references":{"pubmed":["39013974"],"doi":["10.1038/s41598-024-66916-y"]}}