{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(4)"],"submitter":["Li S"],"funding":["Jiangnan University"],"pubmed_abstract":["The diterpene <i>ent</i>-copalol is an important precursor to the synthesis of andrographolide and is found only in green chiretta <i>(Andrographis paniculata)</i>. <i>De novo</i> biosynthesis of <i>ent</i>-copalol has not been reported, because the catalytic activity of <i>ent</i>-copalyl diphosphate synthase (CPS) is very low in microorganisms. In order to achieve the biosynthesis of <i>ent</i>-copalol, <i>Saccharomyces cerevisiae</i> was selected as the chassis strain, because its endogenous mevalonate pathway and dephosphorylases could provide natural promotion for the synthesis of <i>ent</i>-copalol. The strain capable of synthesizing diterpene geranylgeranyl pyrophosphate was constructed by strengthening the mevalonate pathway genes and weakening the competing pathway. Five full-leng"],"journal":["Synthetic and systems biotechnology"],"pagination":["784-792"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11253141"],"repository":["biostudies-literature"],"pubmed_title":["Screening of <i>ent</i>-copalyl diphosphate synthase and metabolic engineering to achieve <i>de novo</i> biosynthesis of <i>ent</i>-copalol in <i>Saccharomyces cerevisiae</i>."],"pmcid":["PMC11253141"],"pubmed_authors":["Yin X","Li S","Lu J","Xu S","Wang X","Luo S","Gao S","Zhao X","Zhou J"],"additional_accession":[]},"is_claimable":false,"name":"Screening of <i>ent</i>-copalyl diphosphate synthase and metabolic engineering to achieve <i>de novo</i> biosynthesis of <i>ent</i>-copalol in <i>Saccharomyces cerevisiae</i>.","description":"The diterpene <i>ent</i>-copalol is an important precursor to the synthesis of andrographolide and is found only in green chiretta <i>(Andrographis paniculata)</i>. <i>De novo</i> biosynthesis of <i>ent</i>-copalol has not been reported, because the catalytic activity of <i>ent</i>-copalyl diphosphate synthase (CPS) is very low in microorganisms. In order to achieve the biosynthesis of <i>ent</i>-copalol, <i>Saccharomyces cerevisiae</i> was selected as the chassis strain, because its endogenous mevalonate pathway and dephosphorylases could provide natural promotion for the synthesis of <i>ent</i>-copalol. The strain capable of synthesizing diterpene geranylgeranyl pyrophosphate was constructed by strengthening the mevalonate pathway genes and weakening the competing pathway. Five full-leng","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2026-07-15T05:13:05.56Z","creation":"2025-04-04T15:02:28.785Z"},"accession":"S-EPMC11253141","cross_references":{"pubmed":["39021361"],"doi":["10.1016/j.synbio.2024.06.005"]}}