biostudies-literatureUnknown9(4)Li SJiangnan UniversityThe diterpene <i>ent</i>-copalol is an important precursor to the synthesis of andrographolide and is found only in green chiretta <i>(Andrographis paniculata)</i>. <i>De novo</i> biosynthesis of <i>ent</i>-copalol has not been reported, because the catalytic activity of <i>ent</i>-copalyl diphosphate synthase (CPS) is very low in microorganisms. In order to achieve the biosynthesis of <i>ent</i>-copalol, <i>Saccharomyces cerevisiae</i> was selected as the chassis strain, because its endogenous mevalonate pathway and dephosphorylases could provide natural promotion for the synthesis of <i>ent</i>-copalol. The strain capable of synthesizing diterpene geranylgeranyl pyrophosphate was constructed by strengthening the mevalonate pathway genes and weakening the competing pathway. Five full-lengSynthetic and systems biotechnology784-792https://www.ebi.ac.uk/biostudies/studies/S-EPMC11253141biostudies-literatureScreening of <i>ent</i>-copalyl diphosphate synthase and metabolic engineering to achieve <i>de novo</i> biosynthesis of <i>ent</i>-copalol in <i>Saccharomyces cerevisiae</i>.PMC11253141Yin XLi SLu JXu SWang XLuo SGao SZhao XZhou JfalseScreening of <i>ent</i>-copalyl diphosphate synthase and metabolic engineering to achieve <i>de novo</i> biosynthesis of <i>ent</i>-copalol in <i>Saccharomyces cerevisiae</i>.The diterpene <i>ent</i>-copalol is an important precursor to the synthesis of andrographolide and is found only in green chiretta <i>(Andrographis paniculata)</i>. <i>De novo</i> biosynthesis of <i>ent</i>-copalol has not been reported, because the catalytic activity of <i>ent</i>-copalyl diphosphate synthase (CPS) is very low in microorganisms. In order to achieve the biosynthesis of <i>ent</i>-copalol, <i>Saccharomyces cerevisiae</i> was selected as the chassis strain, because its endogenous mevalonate pathway and dephosphorylases could provide natural promotion for the synthesis of <i>ent</i>-copalol. The strain capable of synthesizing diterpene geranylgeranyl pyrophosphate was constructed by strengthening the mevalonate pathway genes and weakening the competing pathway. Five full-leng2024-01-01T00:00:00Z2024 Dec2026-07-15T05:13:05.56Z2025-04-04T15:02:28.785ZS-EPMC112531413902136110.1016/j.synbio.2024.06.005