biostudies-literatureLind-Holm Mogensen FFonds National de la Recherche Luxembourg174https://www.ebi.ac.uk/biostudies/studies/S-EPMC11253405biostudies-literatureUnknown21(1)<h4>Background</h4>Specific microglia responses are thought to contribute to the development and progression of neurodegenerative diseases, including Parkinson's disease (PD). However, the phenotypic acquisition of microglial cells and their role during the underlying neuroinflammatory processes remain largely elusive. Here, according to the multiple-hit hypothesis, which stipulates that PD etiology is determined by a combination of genetics and various environmental risk factors, we investigate microglial transcriptional programs and morphological adaptations under PARK7/DJ-1 deficiency, a genetic cause of PD, during lipopolysaccharide (LPS)-induced inflammation.<h4>Methods</h4>Using a combination of single-cell RNA-sequencing, bulk RNA-sequencing, multicolor flow cytometry and immunofluoJournal of neuroinflammationPARK7/DJ-1 deficiency impairs microglial activation in response to LPS-induced inflammation.PMC11253405C19/BM/13688526FNR9631103AFR/6916713PRIDE21/16749720PRIDE/14254520/I2TRONC21/BM/15739125/DIOMEDESINTER/DFG/17/11583046PRIDE/10907093/CRITICSAntony PMANicot NPoovathingal SKTziortziou MPereira SLSkupin AKaoma TBadanjak KWurst WMichelucci AAmeli CCoowar DPoli ANazarov PVLind-Holm Mogensen FGinolhac AMuller AVogt Weisenhorn DMGrunewald ASousa CScafidi AfalsePARK7/DJ-1 deficiency impairs microglial activation in response to LPS-induced inflammation.<h4>Background</h4>Specific microglia responses are thought to contribute to the development and progression of neurodegenerative diseases, including Parkinson's disease (PD). However, the phenotypic acquisition of microglial cells and their role during the underlying neuroinflammatory processes remain largely elusive. Here, according to the multiple-hit hypothesis, which stipulates that PD etiology is determined by a combination of genetics and various environmental risk factors, we investigate microglial transcriptional programs and morphological adaptations under PARK7/DJ-1 deficiency, a genetic cause of PD, during lipopolysaccharide (LPS)-induced inflammation.<h4>Methods</h4>Using a combination of single-cell RNA-sequencing, bulk RNA-sequencing, multicolor flow cytometry and immunofluo2024-01-01T00:00:00Z2024 Jul2025-04-04T15:02:36.848Z2025-04-04T15:02:36.848ZS-EPMC112534053901448210.1186/s12974-024-03164-x