biostudies-literatureYe WHunan Youth Science and Technology Talent ProjectHunan Provincial Natural Science Foundation of ChinaFundamental Research Funds for Central Universities of the Central South UniversityChina Postdoctoral Science Foundatione14850https://www.ebi.ac.uk/biostudies/studies/S-EPMC11255415biostudies-literatureUnknown30(7)<h4>Introduction</h4>Glioma is the most frequent and lethal form of primary brain tumor. The molecular mechanism of oncogenesis and progression of glioma still remains unclear, rendering the therapeutic effect of conventional radiotherapy, chemotherapy, and surgical resection insufficient. In this study, we sought to explore the function of HEC1 (highly expressed in cancer 1) in glioma; a component of the NDC80 complex in glioma is crucial in the regulation of kinetochore.<h4>Methods</h4>Bulk RNA and scRNA-seq analyses were used to infer HEC1 function, and in vitro experiments validated its function.<h4>Results</h4>HEC1 overexpression was observed in glioma and was indicative of poor prognosis and malignant clinical features, which was confirmed in human glioma tissues. High HEC1 expressioCNS neuroscience & therapeuticsNDC80/HEC1 promotes macrophage polarization and predicts glioma prognosis via single-cell RNA-seq and in vitro experiment.PMC112554152020zzts274NO.2018M633002NO.2022JJ20095NO.2023RC3074Chen QHuang YLiang XCheng QZhang HChen GZhang NYe WChen XfalseNDC80/HEC1 promotes macrophage polarization and predicts glioma prognosis via single-cell RNA-seq and in vitro experiment.<h4>Introduction</h4>Glioma is the most frequent and lethal form of primary brain tumor. The molecular mechanism of oncogenesis and progression of glioma still remains unclear, rendering the therapeutic effect of conventional radiotherapy, chemotherapy, and surgical resection insufficient. In this study, we sought to explore the function of HEC1 (highly expressed in cancer 1) in glioma; a component of the NDC80 complex in glioma is crucial in the regulation of kinetochore.<h4>Methods</h4>Bulk RNA and scRNA-seq analyses were used to infer HEC1 function, and in vitro experiments validated its function.<h4>Results</h4>HEC1 overexpression was observed in glioma and was indicative of poor prognosis and malignant clinical features, which was confirmed in human glioma tissues. High HEC1 expressio2024-01-01T00:00:00Z2024 Jul2026-04-08T19:16:46.598Z2025-04-06T17:12:05.746ZS-EPMC112554153902128710.1111/cns.14850