biostudies-literatureUnknown14(6)Wang JThe stimulator of interferon genes (STING), an integral adaptor protein in the DNA-sensing pathway, plays a pivotal role in the innate immune response against infections. Additionally, it presents a valuable therapeutic target for infectious diseases and cancer. We observed that fangchinoline (Fan), a bis-benzylisoquinoline alkaloid (BBA), effectively impedes the replication of vesicular stomatitis virus (VSV), encephalomyocarditis virus (EMCV), influenza A virus (H1N1), and herpes simplex virus-1 (HSV-1) <i>in vitro</i>. Fan treatment significantly reduced the viral load, attenuated tissue inflammation, and improved survival in a viral sepsis mouse model. Mechanistically, Fan activates the antiviral response in a STING-dependent manner, leading to increased expression of interferon (IFN) Journal of pharmaceutical analysis100972https://www.ebi.ac.uk/biostudies/studies/S-EPMC11255895biostudies-literatureFangchinoline induces antiviral response by suppressing STING degradation.PMC11255895Kong LLiang XZhang MWang JLi YXie FHe YXu AJia XFeng WWang XWang YLuo TfalseFangchinoline induces antiviral response by suppressing STING degradation.The stimulator of interferon genes (STING), an integral adaptor protein in the DNA-sensing pathway, plays a pivotal role in the innate immune response against infections. Additionally, it presents a valuable therapeutic target for infectious diseases and cancer. We observed that fangchinoline (Fan), a bis-benzylisoquinoline alkaloid (BBA), effectively impedes the replication of vesicular stomatitis virus (VSV), encephalomyocarditis virus (EMCV), influenza A virus (H1N1), and herpes simplex virus-1 (HSV-1) <i>in vitro</i>. Fan treatment significantly reduced the viral load, attenuated tissue inflammation, and improved survival in a viral sepsis mouse model. Mechanistically, Fan activates the antiviral response in a STING-dependent manner, leading to increased expression of interferon (IFN) 2024-01-01T00:00:00Z2024 Jun2026-06-01T16:29:35.898Z2025-04-04T14:46:54.092ZS-EPMC112558953902791010.1016/j.jpha.2024.100972