biostudies-literatureUnknown8(4)Urano T<h4>Background</h4>Thrombin activatable fibrinolysis inhibitor (TAFI) is one of the most important physiological fibrinolysis inhibitors. Its inhibitory efficacy under physiological conditions remains uncertain.<h4>Objectives</h4>Elucidate the role of soluble thrombomodulin (sTM)/TAFI axis in the regulation of fibrinlysis.<h4>Methods</h4>Since thrombin is required to generate activated TAFI (TAFIa) that targets the C-terminal lysine of partially digested fibrin, a clot lysis assay is suitable for evaluating its function. Using tissue-type plasminogen activator-induced plasma clot lysis time (tPA-PCLT) together with TAFIa inhibitor and recombinant sTM (rsTM), we evaluated the specific function of TM/TAFI in the plasma milieu.<h4>Results</h4>tPA-PCLT values were significantly shortened by thResearch and practice in thrombosis and haemostasis102463https://www.ebi.ac.uk/biostudies/studies/S-EPMC11255936biostudies-literatureEvaluation of thrombomodulin/thrombin activatable fibrinolysis inhibitor function in plasma using tissue-type plasminogen activator-induced plasma clot lysis time.PMC11255936Morooka NSano YHonkura NOkada MSuzuki YDoi MSano HUrano TfalseEvaluation of thrombomodulin/thrombin activatable fibrinolysis inhibitor function in plasma using tissue-type plasminogen activator-induced plasma clot lysis time.<h4>Background</h4>Thrombin activatable fibrinolysis inhibitor (TAFI) is one of the most important physiological fibrinolysis inhibitors. Its inhibitory efficacy under physiological conditions remains uncertain.<h4>Objectives</h4>Elucidate the role of soluble thrombomodulin (sTM)/TAFI axis in the regulation of fibrinlysis.<h4>Methods</h4>Since thrombin is required to generate activated TAFI (TAFIa) that targets the C-terminal lysine of partially digested fibrin, a clot lysis assay is suitable for evaluating its function. Using tissue-type plasminogen activator-induced plasma clot lysis time (tPA-PCLT) together with TAFIa inhibitor and recombinant sTM (rsTM), we evaluated the specific function of TM/TAFI in the plasma milieu.<h4>Results</h4>tPA-PCLT values were significantly shortened by th2024-01-01T00:00:00Z2024 May2025-04-18T13:10:47.208Z2025-04-06T22:45:41.39ZS-EPMC112559363902666010.1016/j.rpth.2024.102463