{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Aher A"],"funding":["NIGMS NIH HHS"],"pagination":["1124-1133"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11257807"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["31(7)"],"pubmed_abstract":["Microtubules are composed of α-tubulin and β-tubulin dimers positioned head-to-tail to form protofilaments that associate laterally in varying numbers. It is not known how cellular microtubules assemble with the canonical 13-protofilament architecture, resulting in micrometer-scale α/β-tubulin tracks for intracellular transport that align with, rather than spiral along, the long axis of the filament. We report that the human ~2.3 MDa γ-tubulin ring complex (γ-TuRC), an essential regulator of microtubule formation that contains 14 γ-tubulins, selectively nucleates 13-protofilament microtubules. Cryogenic electron microscopy reconstructions of γ-TuRC-capped microtubule minus ends reveal the extensive intra-domain and inter-domain motions of γ-TuRC subunits that accommodate luminal bridge com"],"journal":["Nature structural & molecular biology"],"pubmed_title":["Structure of the γ-tubulin ring complex-capped microtubule."],"pmcid":["PMC11257807"],"funding_grant_id":["R35 GM130234"],"pubmed_authors":["Aher A","Kapoor TM","Urnavicius L","Xue A","Neselu K"],"additional_accession":[]},"is_claimable":false,"name":"Structure of the γ-tubulin ring complex-capped microtubule.","description":"Microtubules are composed of α-tubulin and β-tubulin dimers positioned head-to-tail to form protofilaments that associate laterally in varying numbers. It is not known how cellular microtubules assemble with the canonical 13-protofilament architecture, resulting in micrometer-scale α/β-tubulin tracks for intracellular transport that align with, rather than spiral along, the long axis of the filament. We report that the human ~2.3 MDa γ-tubulin ring complex (γ-TuRC), an essential regulator of microtubule formation that contains 14 γ-tubulins, selectively nucleates 13-protofilament microtubules. Cryogenic electron microscopy reconstructions of γ-TuRC-capped microtubule minus ends reveal the extensive intra-domain and inter-domain motions of γ-TuRC subunits that accommodate luminal bridge com","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2026-06-02T01:07:15.551Z","creation":"2026-05-24T03:07:27.279Z"},"accession":"S-EPMC11257807","cross_references":{"pubmed":["38609661"],"doi":["10.1038/s41594-024-01264-z"]}}