{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Prudhomme N"],"funding":["Natural Sciences and Engineering Research Council of Canada","Natural Sciences and Engineering Research Council of Canada (NSERC) - Collaborative Research and Development Grant","J.D. Irving, Limited - Excellence in Cancer Research Fund","Canadian Cancer Society (Atlantic Cancer Research Grant)","Ontario Graduate Scholarship","University of Guelph","Natural Sciences and Engineering Research Council of Canada, Discovery Grant","Mitacs"],"pagination":["2248-2266"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11258984"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(8)"],"pubmed_abstract":["The need for therapeutics to treat a plethora of medical conditions and diseases is on the rise and the demand for alternative approaches to mammalian-based production systems is increasing. Plant-based strategies provide a safe and effective alternative to produce biological drugs but have yet to enter mainstream manufacturing at a competitive level. Limitations associated with batch consistency and target protein production levels are present; however, strategies to overcome these challenges are underway. In this study, we apply state-of-the-art mass spectrometry-based proteomics to define proteome remodelling of the plant following agroinfiltration with bacteria grown under shake flask or bioreactor conditions. We observed distinct signatures of bacterial protein production correspondin"],"journal":["Plant biotechnology journal"],"pubmed_title":["Bacterial growth-mediated systems remodelling of Nicotiana benthamiana defines unique signatures of target protein production in molecular pharming."],"pmcid":["PMC11258984"],"funding_grant_id":["RGPIN-2020-05822","CRDPJ 539389‐19","CRDPJ 539389-19","707196","IT25508"],"pubmed_authors":["Geddes-McAlister J","Sproule A","Krieger JR","Cossar D","Overy DP","Prudhomme N","Thomson S","Pastora R","Murphy JP","McLean MD","Zheng E"],"additional_accession":[]},"is_claimable":false,"name":"Bacterial growth-mediated systems remodelling of Nicotiana benthamiana defines unique signatures of target protein production in molecular pharming.","description":"The need for therapeutics to treat a plethora of medical conditions and diseases is on the rise and the demand for alternative approaches to mammalian-based production systems is increasing. Plant-based strategies provide a safe and effective alternative to produce biological drugs but have yet to enter mainstream manufacturing at a competitive level. Limitations associated with batch consistency and target protein production levels are present; however, strategies to overcome these challenges are underway. In this study, we apply state-of-the-art mass spectrometry-based proteomics to define proteome remodelling of the plant following agroinfiltration with bacteria grown under shake flask or bioreactor conditions. We observed distinct signatures of bacterial protein production correspondin","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Aug","modification":"2026-06-03T03:36:26.027Z","creation":"2025-05-18T13:26:06.231Z"},"accession":"S-EPMC11258984","cross_references":{"pubmed":["38516995"],"doi":["10.1111/pbi.14342"]}}