biostudies-literaturePrudhomme NNatural Sciences and Engineering Research Council of CanadaNatural Sciences and Engineering Research Council of Canada (NSERC) - Collaborative Research and Development GrantJ.D. Irving, Limited - Excellence in Cancer Research FundCanadian Cancer Society (Atlantic Cancer Research Grant)Ontario Graduate ScholarshipUniversity of GuelphNatural Sciences and Engineering Research Council of Canada, Discovery GrantMitacs2248-2266https://www.ebi.ac.uk/biostudies/studies/S-EPMC11258984biostudies-literatureUnknown22(8)The need for therapeutics to treat a plethora of medical conditions and diseases is on the rise and the demand for alternative approaches to mammalian-based production systems is increasing. Plant-based strategies provide a safe and effective alternative to produce biological drugs but have yet to enter mainstream manufacturing at a competitive level. Limitations associated with batch consistency and target protein production levels are present; however, strategies to overcome these challenges are underway. In this study, we apply state-of-the-art mass spectrometry-based proteomics to define proteome remodelling of the plant following agroinfiltration with bacteria grown under shake flask or bioreactor conditions. We observed distinct signatures of bacterial protein production correspondinPlant biotechnology journalBacterial growth-mediated systems remodelling of Nicotiana benthamiana defines unique signatures of target protein production in molecular pharming.PMC11258984RGPIN-2020-05822CRDPJ 539389‐19CRDPJ 539389-19707196IT25508Geddes-McAlister JSproule AKrieger JRCossar DOvery DPPrudhomme NThomson SPastora RMurphy JPMcLean MDZheng EfalseBacterial growth-mediated systems remodelling of Nicotiana benthamiana defines unique signatures of target protein production in molecular pharming.The need for therapeutics to treat a plethora of medical conditions and diseases is on the rise and the demand for alternative approaches to mammalian-based production systems is increasing. Plant-based strategies provide a safe and effective alternative to produce biological drugs but have yet to enter mainstream manufacturing at a competitive level. Limitations associated with batch consistency and target protein production levels are present; however, strategies to overcome these challenges are underway. In this study, we apply state-of-the-art mass spectrometry-based proteomics to define proteome remodelling of the plant following agroinfiltration with bacteria grown under shake flask or bioreactor conditions. We observed distinct signatures of bacterial protein production correspondin2024-01-01T00:00:00Z2024 Aug2026-06-03T03:36:26.027Z2025-05-18T13:26:06.231ZS-EPMC112589843851699510.1111/pbi.14342