{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Qin L"],"funding":["Strategic Priority Research Program of the Chinese Academy of Sciences","MOST | National Key Research and Development Program of China (NKPs)","MOST | National Key Research and Development Program of China","National Natural Science Foundation of China","NINDS NIH HHS","HHS | NIH","International Partnership Program of Chinese Academy of Sciences","HHS | NIH (NIH)"],"pagination":["e2323040121"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11260165"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["121(29)"],"pubmed_abstract":["Stomata in leaves regulate gas (carbon dioxide and water vapor) exchange and water transpiration between plants and the atmosphere. SLow Anion Channel 1 (SLAC1) mediates anion efflux from guard cells and plays a crucial role in controlling stomatal aperture. It serves as a central hub for multiple signaling pathways in response to environmental stimuli, with its activity regulated through phosphorylation via various plant protein kinases. However, the molecular mechanism underlying SLAC1 phosphoactivation has remained elusive. Through a combination of protein sequence analyses, AlphaFold-based modeling and electrophysiological studies, we unveiled that the highly conserved motifs on the N- and C-terminal segments of SLAC1 form a cytosolic regulatory domain (CRD) that interacts with the tra"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Mechanistic insights into phosphoactivation of SLAC1 in guard cell signaling."],"pmcid":["PMC11260165"],"funding_grant_id":["153E11KYSB20190029","NS109366","2021YFA1300702","R01 NS109366","2020YFA0509903","31872721","XDA24020305"],"pubmed_authors":["Zhang XY","Qin L","Deng YN","Su M","Xie Q","Wang K","Xu SM","Zhang XH","Hendrickson WA","Zhang CR","Tang LH","Wang MH","Chen YH"],"additional_accession":[]},"is_claimable":false,"name":"Mechanistic insights into phosphoactivation of SLAC1 in guard cell signaling.","description":"Stomata in leaves regulate gas (carbon dioxide and water vapor) exchange and water transpiration between plants and the atmosphere. SLow Anion Channel 1 (SLAC1) mediates anion efflux from guard cells and plays a crucial role in controlling stomatal aperture. It serves as a central hub for multiple signaling pathways in response to environmental stimuli, with its activity regulated through phosphorylation via various plant protein kinases. However, the molecular mechanism underlying SLAC1 phosphoactivation has remained elusive. Through a combination of protein sequence analyses, AlphaFold-based modeling and electrophysiological studies, we unveiled that the highly conserved motifs on the N- and C-terminal segments of SLAC1 form a cytosolic regulatory domain (CRD) that interacts with the tra","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2026-06-02T02:08:35.054Z","creation":"2026-04-13T03:12:37.735Z"},"accession":"S-EPMC11260165","cross_references":{"pubmed":["38985761"],"doi":["10.1073/pnas.2323040121"]}}