biostudies-literatureMu WShanghai Key Discipline of Public HealthInnovative Research Team of High-level Local Universities in ShanghaiKey laboratory of the Ministry of Education FoundationSanming Project of Medicine in ShenzhenNational Nature Science FoundationShanghai Science and Technology Development Funds718-738https://www.ebi.ac.uk/biostudies/studies/S-EPMC11260768biostudies-literatureUnknown44(7)<h4>Background</h4>Benzo[a]pyrene (B[a]P), a carcinogen pollutant produced by combustion processes, is present in the western diet with grilled meats. Chronic exposure of B[a]P in hepatocellular carcinoma (HCC) cells promotes metastasis rather than primary proliferation, implying an unknown mechanism of B[a]P-induced malignancy. Given that exosomes carry bioactive molecules to distant sites, we investigated whether and how exosomes mediate cancer-stroma communications for a toxicologically associated microenvironment.<h4>Method</h4>Exosomes were isolated from B[a]P stimulated BEL7404 HCC cells (7404-100Bap Exo) at an environmental relevant dose (100 nmol/L). Lung pre-education animal model was prepared via injection of exosomes and cytokines. The inflammatory genes of educated lungs were eCancer communications (London, England)Exposure of benzo[a]pyrene induces HCC exosome-circular RNA to activate lung fibroblasts and trigger organotropic metastasis.PMC11260768SHSMU-ZLCX20211602SZSM2023110192022-MEKLLC-MS-00323QA14057008217354381902939GWVI-11.1-20Li HGe YJia WMu WGu PJian YZhou JfalseExposure of benzo[a]pyrene induces HCC exosome-circular RNA to activate lung fibroblasts and trigger organotropic metastasis.<h4>Background</h4>Benzo[a]pyrene (B[a]P), a carcinogen pollutant produced by combustion processes, is present in the western diet with grilled meats. Chronic exposure of B[a]P in hepatocellular carcinoma (HCC) cells promotes metastasis rather than primary proliferation, implying an unknown mechanism of B[a]P-induced malignancy. Given that exosomes carry bioactive molecules to distant sites, we investigated whether and how exosomes mediate cancer-stroma communications for a toxicologically associated microenvironment.<h4>Method</h4>Exosomes were isolated from B[a]P stimulated BEL7404 HCC cells (7404-100Bap Exo) at an environmental relevant dose (100 nmol/L). Lung pre-education animal model was prepared via injection of exosomes and cytokines. The inflammatory genes of educated lungs were e2024-01-01T00:00:00Z2024 Jul2026-07-14T20:36:00.157Z2025-04-04T14:46:02.924ZS-EPMC112607683884055110.1002/cac2.12574