biostudies-literatureYang XNHLBI NIH HHSNINDS NIH HHSNIGMS NIH HHS1836-1843https://www.ebi.ac.uk/biostudies/studies/S-EPMC11260907biostudies-literatureUnknown42(12)Realizing the full potential of organoids and assembloids to model neural development and disease will require improved methods for long-term, minimally invasive recording of electrical activity. Current technologies, such as patch clamp, penetrating microelectrodes, planar electrode arrays and substrate-attached flexible electrodes, do not allow chronic recording of organoids in suspension, which is necessary to preserve architecture. Inspired by kirigami art, we developed flexible electronics that transition from a two-dimensional to a three-dimensional basket-like configuration with either spiral or honeycomb patterns to accommodate the long-term culture of organoids in suspension. Here we show that this platform, named kirigami electronics (KiriE), integrates with and enables chronic rNature biotechnologyKirigami electronics for long-term electrophysiological recording of human neural organoids and assembloids.PMC11260907R35 GM141598R01 NS121934R01 HL165491Li TLTsai CTPasca SPMollo VSantoro FKanton SYang XForro CMiura YMcQueen JPZaluska TJChen XCui BfalseKirigami electronics for long-term electrophysiological recording of human neural organoids and assembloids.Realizing the full potential of organoids and assembloids to model neural development and disease will require improved methods for long-term, minimally invasive recording of electrical activity. Current technologies, such as patch clamp, penetrating microelectrodes, planar electrode arrays and substrate-attached flexible electrodes, do not allow chronic recording of organoids in suspension, which is necessary to preserve architecture. Inspired by kirigami art, we developed flexible electronics that transition from a two-dimensional to a three-dimensional basket-like configuration with either spiral or honeycomb patterns to accommodate the long-term culture of organoids in suspension. Here we show that this platform, named kirigami electronics (KiriE), integrates with and enables chronic r2024-01-01T00:00:00Z2024 Dec2025-04-26T16:11:04.781Z2025-04-06T15:07:24.211ZS-EPMC112609073825388010.1038/s41587-023-02081-3